Solute Carrier Family 27 Member 6 (SLC27A6) Possibly Promotes the Proliferation of Papillary Thyroid Cancer by Regulating c-MYC

被引：3

作者：

Liu, Changjian ^{[1
]}

Wang, Jian ^{[1
]}

Li, Dongdong ^{[2
]}

Ni, Ruoxuan ^{[2
]}

Zhao, Mei ^{[2
]}

Huang, ChangZhi ^{[2
]}

Liu, Shaoyan ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Canc Hosp, Natl Clin Res Ctr Canc, Dept Head & Neck Surg,Natl Canc Ctr, Beijing 100021, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Canc Hosp, Natl Clin Res Ctr Canc, Dept Etiol & Carcinogenesis,Natl Canc Ctr, Beijing 100021, Peoples R China

来源：

BIOCHEMICAL GENETICS | 2022年 / 60卷 / 06期

关键词：

Papillary thyroid cancer; c-MYC; BRAF mutations; FATTY-ACID TRANSPORT; METABOLISM; MEMBRANE; GROWTH;

D O I：

10.1007/s10528-022-10218-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the expression and mechanism of LSC27A6 in papillary thyroid cancer (PTC). We analyzed the differential expression of SLC27A6 in PTC tissues and normal tissues based on the TCGA database and validated it using immunohistochemistry. Wilcoxon rank sum, chi-square test, or Fisher exact exam were used to analyze the relationship between the expression of SLC27A6 and clinicopathological information. Samples were divided into two groups according to whether BRAF was mutated or not, and Wilcoxon rank sum was used to determine whether the expression of SLC27A6 was related to BRAF mutation. The effects of SLC27A6 on the proliferation, migration, and apoptosis of PTC cells were detected by cell counting kit-8 (CCK8), colony formation assay, transwell assay, and flow cytometry. Spearman correlation analysis was used to evaluate the relationship between SLC27A6 and c-MYC. Protein expression was detected by Western blot. The expression of SLC27A6 was higher in PTC and positively correlated with N stage. SLC27A6 expression was higher in samples with BRAF mutations. Down-regulation of SLC27A6 inhibited cell proliferation, migration, and invasion and induced apoptosis. Spearman correlation analysis showed that SLC27A6 was positively correlated with c-MYC. Knockdown of SLC27A6 inhibited c-MYC expression. Our results suggest that SLC27A6 is overexpressed in PTC tissues and affects the progression of PTC by regulating c-MYC.

引用

页码：2313 / 2326

页数：14

共 43 条

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