In Vivo Screening for Anti-Osteoporotic Fraction from Extract of Herbal Formula Xianlinggubao in Ovariectomized Mice

被引：46

作者：

Wang, Xinluan ^{[1
,2
]}

He, Yixin ^{[2
]}

Guo, Baosheng ^{[2
]}

Tsang, Man-Ching ^{[2
]}

Tu, Fengjuan ^{[3
]}

Dai, Yi ^{[3
]}

Yao, Zhihong ^{[3
]}

Zheng, Lizhen ^{[2
]}

Xie, Xinhui ^{[2
]}

Wang, Nan ^{[1
]}

Yao, Xinsheng ^{[3
]}

Zhang, Ge ^{[2
]}

Qin, Ling ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Translat Med R&D Ctr, Shenzhen Inst Adv Technol, Inst Biomed Engn & Hlth Tec, Shenzhen 518000, Peoples R China

[2] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China

[3] Jinan Univ, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 02期

基金：

中国国家自然科学基金;

关键词：

POSTMENOPAUSAL OSTEOPOROSIS; BONE LOSS; BIOCHEMICAL MARKERS; CONTROLLED TRIAL; TOTAL SAPONINS; RADIX-DIPSACI; RATS; FLAVONOIDS; DIFFERENTIATION; IDENTIFICATION;

D O I：

10.1371/journal.pone.0118184

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background and Objectives Traditional Chinese Medicine (TCM) Fufang or formula Xianlinggubao (XLGB) is a prescribed TCM drug in China registered for prevention and treatment of osteoporosis. Fufang in TCM is comprised of a group of herbal compounds contributing in group to the treatment efficacy. The present study aims to identify the bioactive fraction(s) in XLGB extract that account(s) dominantly for its osteogenic effects. Methods The extract of XLGB formula was separated into three fractions using chromatography, i.e., XLGB-A, XLGB-B and XLGB-C. They were administrated to 4-month old ovariectomized (OVX) mice for 6 weeks to determine which bioactive fraction(s) were more effective for preventing OVX-induced bone loss evaluated by microCT, biomechanical testing and biochemical markers. The main peaks of the key fraction were identified using reference compounds isolated from the fraction. In addition, the effects of the composite compounds in XLGB-B on osteoblasts' proliferation and mineralization were evaluated in UMR 106 cells. Results XLGB-B with a yield of 13.0% from herbal Fufang XLGB was identified as the most potential one among the three fractions for prevention of OVX-induced bone loss confirmed with bone mass, bonemicroarchitecture, bone strength and bone turnover markers. Nine compounds in HPLC fingerprint were identified in the XLGB-B fraction, including phenylpropanoids from Herba Epimedii, terpenes from Radix Dipsaci and coumarins from Fructus Psoraleae. In addition, the identified compounds effectively promoted proliferation and/or mineralization of osteoblast-like UMR 106 cells in vitro. Conclusion XLGB-B with defined phytochemical structures was screened as the key fraction that demonstrated preventive effects on OVX-induced bone loss in mice. The present study laid down a foundation towards a new generation of herbal Fufang characterized with "less herbal materials for achieving equal treatment efficacy" in development strategy of TCM for prevention of OVX-induced osteoporosis.

引用

页数：17

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