Validation of a Five Plate Test, the STAR protocol, for the screening of antibiotic residues in muscle from different animal species according to European Decision 2002/657/EC

被引：35

作者：

Gaudin, V. ^{[1
]}

Hedou, C. ^{[1
]}

Rault, A. ^{[1
]}

Verdon, E. ^{[1
]}

机构：

[1] AFSSA Fougeres LERMVD, Community Reference Lab, F-35302 Fougere, France

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2010年 / 27卷 / 07期

关键词：

in-house validation; screening-microbial screening; residues; antibiotics; antimicrobials; veterinary drugs; meat; KIDNEY; MEAT; IDENTIFICATION; SAMPLES; TISSUE; ASSAY;

D O I：

10.1080/19440041003677483

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The STAR protocol is a Five Plate Test (FPT) developed several years ago at the Community Reference Laboratory (CRL) for the screening of antimicrobial residues in milk and muscle. This paper presents the validation of this method according to European Decision 2002/657/EC and to an internal guideline for validation. A validation protocol based on 'simulated tissues' and on a list of 16 representative antimicrobials to be validated was implemented in our laboratory during several months for the STAR protocol. The performance characteristics of the method were determined (specificity, detection capabilities CC, applicability, ruggedness). In conclusion, the STAR protocol is applicable to the broad-spectrum detection of antibiotic residues in muscles of different animal species (pig, cattle, sheep, poultry). The method has good specificity (false-positive rate = 4%). The detection capabilities were determined for 16 antibiotics from different families in relation to their respective maximum residue limit (MRL): beta-lactams (penicillins and cephalosporins MRL), tetracyclines (MRL and 2.5 MRL), macrolides (2 MRL), quinolones (2 MRL), some sulphonamides (3 MRL), and trimethoprim (2 MRL). However, the sensitivity of the STAR protocol towards aminoglycosides (8 MRL) and florfenicol (10 MRL) was unsatisfactory (MRL). The two objectives of this study were met: firstly, to validate the STAR protocol according to European Decision 2002/657/EC, then to demonstrate that the validation guideline developed to implement this decision is applicable to microbiological plate tests even for muscle. The use of simulated tissue appeared a good compromise between spiked discs with antibiotic solutions and incurred tissues. In addition, the choice of a list of representative antibiotics allowed the reduction of the scope of the validation, which was already costly in time and effort.

引用

页码：935 / 952

页数：18

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