Optimization of Extraction Process of Exopolysaccharide from Lentinus edodes by Response Surface Methodology

In this paper, response surface methodology was used to study the optimal extraction parameters of extracellular polysaccharide from Lentinus edodes on the basis of single factor analysis. The factors of the extraction process of lentinan extracellular polysaccharide were optimized and analyzed, and the optimal extraction process of lentinan extracellular polysaccharide was finally determined. That is, the ethanol concentration was 78.0%, the alcohol precipitation time was 15.8 h, and the pH was 7.8. Under the above optimized conditions, the actual extraction amount of extracellular polysaccharides of Lentinus edodes can be increased to 1562.3mg/L, and the stability and reliability are high. The experimental values are roughly consistent with the predicted values of the theoretical linear regression model constructed in the previous period.