Circular RNA hsa_circ_0000554 promotes progression and elevates radioresistance through the miR-485-5p/fermitin family members 1 axis in esophageal cancer

被引:23
作者
Wang, Jianhua [1 ]
Chen, Yushan [2 ]
Wu, Rui [3 ]
Lin, Yong [4 ]
机构
[1] Taizhou Hosp Zhejiang Prov, Dept Radiotherapy, Taizhou, Peoples R China
[2] Fujian Med Univ, Zhangzhou Affiliated Hosp, Dept Radiol, Zhangzhou, Fujian, Peoples R China
[3] Houma Peoples Hosp, Dept Gastrointestinal Surg, Linfen, Shanxi, Peoples R China
[4] Fujian Med Univ, Zhangzhou Affiliated Hosp, Dept Cardiothorac Surg, 59 Shengli West Rd, Zhangzhou 363000, Fujian, Peoples R China
关键词
circ_0000554; esophageal cancer; fermitin family members 1; miR-485-5p; radiotherapy;
D O I
10.1097/CAD.0000000000001007
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Esophageal cancer is one of the most aggressive malignant cancers in the world. Circular RNA hsa_circ_0000554 (circ_0000554) and Fermitin family members 1 (FERMT1) are rated to the advancement of esophageal cancer. Nevertheless, the regulatory mechanisms between circ_0000554 and FERMT1 in the radioresistance of esophageal cancer are unclear. Quantitative real-time PCR (qRT-PCR) was applied to examine the expression of circ_0000554, FERMT1 mRNA, and miR-485-5p. Western blot analysis was employed to assess the protein expression levels of FERMT1, Ki-67, matrix metalloproteinase (MMP)-9 and MMP-2. Cell colony formation, migration, invasion, radiosensitivity and apoptosis were evaluated by cell colony formation, transwell or flow cytometry assays. The relationship between circ_0000554 or FERMT1 and miR-485-5p was verified with dual-luciferase reporter assay. Circ_0000554 and FERMT1 expression was enhanced in esophageal cancer tissues and radioresistant esophageal cancer tissues. Both circ_0000554 and FERMT1 repression blocked cell colony formation, migration, invasion and elevated cell radiosensitivity and apoptosis in esophageal cancer cells. Importantly, circ_0000554 served as a sponge for miR-485-5p in esophageal cancer cells. And FERMT1 acted as a downstream target for miR-485-5p. Additionally, circ_0000554 modulated FERMT1 expression via miR-485-5p. Furthermore, FERMT1 enhancement reversed circ_0000554 inhibition-mediated effects on the colony formation, migration, invasion, radiosensitivity and apoptosis of esophageal cancer cells. Circ_0000554 silencing repressed EC progression and enhanced cell radiosensitivity through downregulating FERMT1 via sponging miR-485-5p, which provided a possible method for improving the radioresistence of esophageal cancer.
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收藏
页码:405 / 416
页数:12
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