Metabolic engineering of Escherichia coli for the production of isoprenoids

被引:55
作者
Ward, Valerie C. A. [1 ,2 ]
Chatzivasileiou, Alkiviadis O. [1 ]
Stephanopoulos, Gregory [1 ]
机构
[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[2] Univ Waterloo, Dept Chem Engn, 200 Univ Ave W, Waterloo, ON N2L 3G1, Canada
关键词
metabolic engineering; isoprenoids; Escherichia coli; mevalonate pathway; methylerythritol pathway; genetic engineering; HETEROLOGOUS MEVALONATE PATHWAY; LYCOPENE PRODUCTION; PHOSPHATE-PATHWAY; HIGH-THROUGHPUT; BETA-CAROTENE; WILD-TYPE; BIOSYNTHESIS; EXPRESSION; SYNTHASE; OVERPRODUCTION;
D O I
10.1093/femsle/fny079
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Metabolic engineering is the practice of using directed genetic manipulations to rewire cellular metabolism primarily with the aim to transform the organism into a single-celled chemical factory. Using biological processes, we can produce more complex chemicals in a more sustainable way. This is particularly important for chemicals which are hard to synthesize using traditional chemistry. However, cells have evolved for growth and must be engineered to produce a single chemical at commercially viable levels. This review focuses on the strategies used to rewire cellular metabolism to produce chemicals using isoprenoid production in Escherichia coli as an example that illustrates many of the challenges faced inmetabolic engineering.
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页数:9
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共 98 条
[1]   Terpenoids: Opportunities for biosynthesis of natural product drugs using engineered microorganisms [J].
Ajikumar, Parayil Kumaran ;
Tyo, Keith ;
Carlsen, Simon ;
Mucha, Oliver ;
Phon, Too Heng ;
Stephanopoulos, Gregory .
MOLECULAR PHARMACEUTICS, 2008, 5 (02) :167-190
[2]   Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli [J].
Ajikumar, Parayil Kumaran ;
Xiao, Wen-Hai ;
Tyo, Keith E. J. ;
Wang, Yong ;
Simeon, Fritz ;
Leonard, Effendi ;
Mucha, Oliver ;
Phon, Too Heng ;
Pfeifer, Blaine ;
Stephanopoulos, Gregory .
SCIENCE, 2010, 330 (6000) :70-74
[3]   Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production [J].
Alonso-Gutierrez, Jorge ;
Chan, Rossana ;
Batth, Tanveer S. ;
Adams, Paul D. ;
Keasling, Jay D. ;
Petzold, Christopher J. ;
Lee, Taek Soon .
METABOLIC ENGINEERING, 2013, 19 :33-41
[4]   Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli [J].
Alper, H ;
Jin, YS ;
Moxley, JF ;
Stephanopoulos, G .
METABOLIC ENGINEERING, 2005, 7 (03) :155-164
[5]   Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene [J].
Anthony, Jennifer R. ;
Anthony, Larry C. ;
Nowroozi, Farnaz ;
Kwon, Gina ;
Newman, Jack D. ;
Keasling, Jay D. .
METABOLIC ENGINEERING, 2009, 11 (01) :13-19
[6]  
Bach TJ, 2000, EUR J BIOCHEM, V1, P103
[7]   Cloning of three A-type cytochromes p450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome p450 in the biosynthesis of the cyanogenic glucoside dhurrin [J].
Bak, S ;
Kahn, RA ;
Nielsen, HL ;
Moller, BL ;
Halkier, BA .
PLANT MOLECULAR BIOLOGY, 1998, 36 (03) :393-405
[8]   Feedback Inhibition of Deoxy-D-xylulose-5-phosphate Synthase Regulates the Methylerythritol 4-Phosphate Pathway [J].
Banerjee, Aparajita ;
Wu, Yan ;
Banerjee, Rahul ;
Li, Yue ;
Yan, Honggao ;
Sharkey, Thomas D. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (23) :16926-16936
[9]   2C-Methyl-D-erythritol 4-Phosphate Enhances and Sustains Cyclodiphosphate Synthase IspF Activity [J].
Bitok, J. Kipchirchir ;
Meyers, Caren Freel .
ACS CHEMICAL BIOLOGY, 2012, 7 (10) :1702-1710
[10]   Computational identification of gene over-expression targets for metabolic engineering of taxadiene production [J].
Boghigian, Brett A. ;
Armando, John ;
Salas, Daniel ;
Pfeifer, Blaine A. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (05) :2063-2073