Microbiology quality, detection of enterotoxin genes and antimicrobial resistance of Staphylococcus aureus isolated from milk and Coalho cheese

被引：7

作者：

Muniz Pereira, Cecilia Teresa ^{[1
]}

Vasconcelos Oliveira, Diego Savio ^{[1
]}

Solano Veloso, Vanessa ^{[2
]}

Paulino Silva, Sabina dos Santos ^{[3
]}

Santos, Leidiane Sousa ^{[4
]}

Lima Neto, Anisio Ferreira ^{[5
]}

de Alcantara Oliveira, Felipe Araujo ^{[6
]}

Nunes de Melo, Maria Celeste ^{[7
]}

dos Santos Soaress, Maria Jose ^{[8
]}

机构：

[1] Univ Fed Piaui, Ctr Ciencias Saude, UFPI, Dept Nutr,PPGAN,Curso Mestrado, Campus Ininga, Teresina, PI, Brazil

[2] Univ Fed Piaui, Ctr Ciencias Agr, Curso Grad Med Vet, Campus Socopo, Teresina, PI, Brazil

[3] Univ Fed Rio Grande do Norte, Ctr Biociencias, Curso Grad Biomed, UFRN, Campus Univ Lagoa Nova, Natal, RN, Brazil

[4] Univ Fed Piaui, Ctr Ciencias Agr, Curso Mestrado, Programa Posgrad Ciencia Anim, Campus Socopo, Teresina, PI, Brazil

[5] EMBRAPA Meio Norte, Empresa Brasileira Pesquisa Agr, Teresina, PI, Brazil

[6] Univ Fed Piaui, Ctr Ciencias Agr, Dept Morfofisiol Vet, Biomed,Ctr Ciencias,Agr, Campus Socopo, Teresina, PI, Brazil

[7] Univ Fed Rio Grande do Norte, Dept Microbiol & Parasitol, Ctr Biociencias, Natal, RN, Brazil

[8] Univ Fed Piaui, Dept Morfofisiol Vet, Ctr Ciencias Agr, Campus Socopo, Teresina, PI, Brazil

来源：

SEMINA-CIENCIAS AGRARIAS | 2018年 / 39卷 / 05期

关键词：

Antimicrobial resistance; Dairy products; Coalho cheese; Staphylococcal enterotoxins genes; Staphylococcus aureus; INDUCIBLE CLINDAMYCIN RESISTANCE; DAIRY-PRODUCTS; METHICILLIN-RESISTANCE; EXFOLIATIVE TOXINS; MASTITIS; OUTBREAK; BRAZIL; FOOD; RAW; PCR;

D O I：

10.5433/1679-0359.2018v39n5p1957

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This study evaluated the microbiological quality of milk and Coalho cheese, the prevalence of enterotoxin genes, antimicrobial resistance and determined an inducible MLSB resistance phenotype by the D-test in strains of Staphylococcus aureus isolated from these products. Seventy samples of milk and Coalho cheese were analyzed. S. aureus strains were identified by biochemical tests. The presence of se genes (sea-see) was tested by polymerase chain reaction. The antimicrobial sensitivity of S. aureus strains was evaluated for 13 antimicrobial drugs using the disk diffusion technique and the double-disk diffusion test (D-test) was performed to determine inducible resistance to lincosamide phenotype. The amount of toxin sufficient to cause foodborne diseases is generally observed when Staphylococcus populations exceed 105 CFU mL(-1) g(-1). In this study, none of the milk samples analyzed showed these counts; however, 73.3% (22/30) of Coalho cheese samples exceeded this value. A total of 109 isolates were identified as S. aureus. The presence of enterotoxin genes was detected in 25.7% of these isolates and amplified only for the sec gene. Most of the isolates (78.5%) were resistant to one or more antimicrobial agents. The D test showed that 25.0% of erythromycin-resistant isolates had the constitutive resistance phenotype, and 3.8% had the inducible resistance phenotype to clindamycin. These results indicate that these dairy products represent a health risk since these bacteria can cause foodborne diseases or may be a possible route for the transfer of antimicrobial resistance to humans.

引用

页码：1957 / 1968

页数：12

共 41 条

[1] Detection of Antibiotic Resistant Staphylococcus aureus from Milk: A Public Health Implication [J].