Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets

被引：29

作者：

Chander, Yogesh ^{[1
]}

Jindal, Naresh ^{[1
]}

Stallknecht, David E. ^{[2
]}

Sreevatsan, Srinand ^{[1
]}

Goyal, Sagar M. ^{[1
]}

机构：

[1] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA

[2] Univ Georgia, SE Cooperat Wildlife Dis Study, Dept Populat Hlth, Coll Vet Med, Athens, GA 30602 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 165卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Antiviral resistance; Avian influenza virus; Gene amplification; Influenza A virus; Neuraminidase gene; Reverse transcription-polymerase chain reaction (RT-PCR); Subtyping; REVERSE-TRANSCRIPTION-PCR; INHIBITION TEST; ASSAY; AMPLIFICATION;

D O I：

10.1016/j.jviromet.2010.01.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An RT-PCR based method was developed using subtype specific overlapping primers to obtain full length amplification of neuraminidase (NA) gene from all subtypes (N1-N9) of influenza A viruses. This method was validated using reference strains of avian influenza viruses (AIV) (N1-N9), human influenza viruses (N1 and N2), and swine influenza viruses (N1-N3). Amplification of the NA gene was obtained with all viruses tested. Additionally, 200 field isolates of AIV from wild birds were tested by this method and the NA gene was amplified in all isolates. The NA subtype of all 200 isolates was determined by further sequencing of the amplified NA genes and all sequences were submitted to GenBank. The method described in this paper can be used to determine subtype of influenza isolates as well as their evolution and mutations if any, in the NA gene. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：116 / 120

页数：5

共 20 条

[1] STANDARD HEMAGGLUTINATION INHIBITION TEST FOR NEWCASTLE-DISEASE .1. COMPARISON OF MACRO AND MICRO METHODS [J].

ALLAN, WH ;

GOUGH, RE .

VETERINARY RECORD, 1974, 95 (06) :120-123

[2] A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus [J].

Alvarez, Alejandra Castillo ;

Brunck, Marion E. G. ;

Boyd, Victoria ;

Lai, Richard ;

Virtue, Elena ;

Chen, Wenbin ;

Bletchly, Cheryl ;

Heine, Hans G. ;

Barnard, Ross .

VIROLOGY JOURNAL, 2008, 5 (1)

[3]

AYMARDHENRY M, 1973, B WORLD HEALTH ORGAN, V48, P199

[4] Animal and human health implications of avian influenza infections [J].

Capua, Ilaria ;

Alexander, Dennis J. .

BIOSCIENCE REPORTS, 2007, 27 (06) :359-372

[5] Rapid molecular subtyping by reverse transcription polymerase chain reaction of the neuraminidase gene of avian influenza A viruses [J].

Fereidouni, S. R. ;

Starick, E. ;

Grund, C. ;

Globig, A. ;

Mettenleiter, T. C. ;

Beer, M. ;

Harder, T. .

VETERINARY MICROBIOLOGY, 2009, 135 (3-4) :253-260

[6] Characterization of a novel influenza a virus hemagglutinin subtype (H16) obtained from black-headed gulls [J].

Fouchier, RAM ;

Munster, V ;

Wallensten, A ;

Bestebroer, TM ;

Herfst, S ;

Smith, D ;

Rimmelzwaan, GF ;

Olsen, B ;

Osterhaus, ADME .

JOURNAL OF VIROLOGY, 2005, 79 (05) :2814-2822

[7] Evaluation of a multiplex real-time reverse transcriptase PCR assay for detection and differentiation of influenza viruses A and B during the 2001-2002 influenza season in Israel [J].

Hindiyeh, M ;

Levy, V ;

Azar, R ;

Varsano, N ;

Regev, L ;

Shalev, Y ;

Grossman, Z ;

Mendelson, E .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) :589-595

[8] Universal primer set for the full-length amplification of all influenza A viruses [J].

Hoffmann, E ;

Stech, J ;

Guan, Y ;

Webster, RG ;

Perez, DR .

ARCHIVES OF VIROLOGY, 2001, 146 (12) :2275-2289

[9] Amplification of four genes of influenza A viruses using a degenerate primer set in a one step RT-PCR method [J].

Jindal, Naresh ;

Chander, Yogesh ;

de Abin, Martha ;

Sreevatsan, Srinand ;

Stallknecht, David ;

Halvorson, David A. ;

Goyal, Sagar M. .

JOURNAL OF VIROLOGICAL METHODS, 2009, 160 (1-2) :163-166

[10] Simultaneous one-tube full-length amplification of the NA, NP, M, and NS genes of influenza A viruses for reverse genetics [J].

Kreibich, Anne ;

Stech, Juergen ;

Mettenleiter, Thomas C. ;

Stech, Olga .

JOURNAL OF VIROLOGICAL METHODS, 2009, 159 (02) :308-310

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