Chemo-biological mRNA imaging with single nucleotide specificity

被引：13

作者：

Knoll, Andrea ^{[1
]}

Kankowski, Svenja ^{[2
]}

Schoellkopf, Sophie ^{[1
]}

Meier, Jochen C. ^{[2
]}

Seitz, Oliver ^{[1
]}

机构：

[1] Humboldt Univ, Dept Chem, Brook Taylor Str 2, D-12489 Berlin, Germany

[2] Tech Univ Carolo Wilhelmina Braunschweig, Zool Inst, Spielmannstr 7, D-38106 Braunschweig, Germany

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 98期

关键词：

PEPTIDE NUCLEIC-ACIDS; FIT-PROBES; THIAZOLE ORANGE; FLUORESCENT BASE; LIVING CELLS; CANCER-CELLS; HYBRIDIZATION; BRIGHTNESS; EXPRESSION; MARKER;

D O I：

10.1039/c9cc06989e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Unambiguous imaging of C -> U edited mRNA calls for a method that distinguishes a locally high concentration of unbound probe or single nucleotide mismatched target from a locally low concentration of matched mRNA target. To address this issue, we combine FIT probes as a "chemical" detection system with the "biological" MS2 technique. Ratio measurements provide a convenient parameter to discriminate the edited from the unedited state of mRNA encoding for GlyR alpha 2 in HEK cells.

引用

页码：14817 / 14820

页数：4

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