Dihydroactinidiolide regulates Nrf2/HO-1 expression and inhibits caspase-3/Bax pathway to protect SH-SY5Y human neuroblastoma cells from oxidative stress induced neuronal apoptosis

被引:30
作者
Das, Mamali [1 ]
Devi, Kasi Pandima [1 ]
机构
[1] Alagappa Univ, Dept Biotechnol, Sci Campus, Karaikkudi 630003, Tamil Nadu, India
关键词
Alzheimer’ s disease; Caspase-3; Dihydroactinidiolide; HO-1; Nrf2; Oxidative stress; SH-SY5Y; ALZHEIMERS-DISEASE; INDUCED TOXICITY; COGNITIVE IMPAIRMENT; AMYLOID-BETA; GLUTAMATE; MODEL; NRF2; PC12; INVOLVEMENT; ACTIVATION;
D O I
10.1016/j.neuro.2021.02.006
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Alzheimer's disease (AD) etiology has been studied for a long time and it is found to be multifaceted involving the accumulation of amyloid beta and tau protein. Oxidative stress is an early event in AD associated neurodegeneration provoking neuronal death through mitochondrial dysfunction and activation of caspase-3. Therefore we tested the efficacy of dihydroactinidiolide (DHAc), a monoterpene lactone against the oxidative load involved in AD like pathological conditions induced by sodium dithionite, glutamate, amyloid beta and colchicine in SH-SY5Y cells. Some of the indicators of neurotoxicity like acetylcholinesterase activity, intracellular reactive oxygen species (ROS), nitrite content, lipid peroxidation, protein carbonylation, nuclear and membrane damage were found to be significantly high in the toxicant treated cells when compared to the control cells while DHAc pretreatment significantly restored the toxicant induced neuronal damage signatures. Caspase-3 activity was found to be increased in the toxicant treated cells while DHAc significantly reduced it. Western blotting and RTPCR revealed that DHAc significantly increased anti-apoptotic Bcl-2 expression and mRNA levels of Nrf2 and HO1. Therefore DHAc was found to protect SH-SY5Y cells from neurotoxicant induced oxidative stress and apoptosis by regulating cellular antioxidant defenses and apoptosis related genes.
引用
收藏
页码:53 / 63
页数:11
相关论文
共 65 条
[1]   Alzheimer's Disease and Parkinson's Disease: A Nutritional Toxicology Perspective of the Impact of Oxidative Stress, Mitochondrial Dysfunction, Nutrigenomics and Environmental Chemicals [J].
Agnihotri, Aayushi ;
Aruoma, Okezie I. .
JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 2020, 39 (01) :16-27
[2]   Induction of Apoptosis and Antiproliferative Activity of Naringenin in Human Epidermoid Carcinoma Cell through ROS Generation and Cell Cycle Arrest [J].
Ahamad, Md Sultan ;
Siddiqui, Sahabjada ;
Jafri, Asif ;
Ahmad, Sheeba ;
Afzal, Mohammad ;
Arshad, Md .
PLOS ONE, 2014, 9 (10)
[3]   Genetic activation of Nrf2 signaling is sufficient to ameliorate neurodegenerative phenotypes in a Drosophila model of Parkinson's disease [J].
Barone, Maria Cecilia ;
Sykiotis, Gerasimos P. ;
Bohmann, Dirk .
DISEASE MODELS & MECHANISMS, 2011, 4 (05) :701-707
[4]   Fingerprint of black teas from India: identification of the regio-specific characteristics [J].
Borse, BB ;
Rao, LJM ;
Nagalakshmi, S ;
Krishnamurthy, N .
FOOD CHEMISTRY, 2002, 79 (04) :419-424
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   Oxidative stress in alzheimer's disease: A review on emergent natural polyphenolic therapeutics [J].
Cassidy, Luke ;
Fernandez, Francesca ;
Johnson, Joel B. ;
Naiker, Mani ;
Owoola, Akeem G. ;
Broszczak, Daniel A. .
COMPLEMENTARY THERAPIES IN MEDICINE, 2020, 49
[7]  
CATALAN R E, 1986, Neuroscience Research, V3, P395, DOI 10.1016/0168-0102(86)90031-3
[8]   Intracellular Generation of ROS by 3,5-Dimethylaminophenol: Persistence, Cellular Response, and Impact of Molecular Toxicity [J].
Chao, Ming-Wei ;
Erkekoglu, Pinar ;
Tseng, Chia-Yi ;
Ye, Wenjie ;
Trudel, Laura J. ;
Skipper, Paul L. ;
Tannenbaum, Steven R. ;
Wogan, Gerald N. .
TOXICOLOGICAL SCIENCES, 2014, 141 (01) :300-313
[9]   Effect of Tacrine-3-caffeic Acid, A Novel Multifunctional Anti-Alzheimer's Dimer, Against Oxidative-Stress-Induced Cell Death in HT22 Hippocampal Neurons: Involvement of Nrf2/HO-1 Pathway [J].
Chao, Xiao-Juan ;
Chen, Zi-Wei ;
Liu, An-Min ;
He, Xi-Xin ;
Wang, Shao-Gui ;
Wang, Yu-Ting ;
Liu, Pei-Qing ;
Ramassamy, Charles ;
Mak, Shing-Hung ;
Cui, Wei ;
Kong, Ah-Ng ;
Yu, Zhi-Ling ;
Han, Yi-Fan ;
Pi, Rong-Biao .
CNS NEUROSCIENCE & THERAPEUTICS, 2014, 20 (09) :840-850
[10]   Recapitulating physiological normoxia in vitro to discriminate Nrf2 regulated gene transcription [J].
Chapple, Sarah J. ;
Keeley, Thomas P. ;
Siow, Richard C. M. ;
Mann, Giovanni E. .
FREE RADICAL BIOLOGY AND MEDICINE, 2017, 108 :S5-S6