An IL-6/IL-6 soluble receptor (IL-6R) hybrid protein (H-IL-6) induces epo-independent erythroid differentiation in human CD34+ cells

被引：7

作者：

Baiocchi, M

Marcucci, I

Rose-John, S

Serlupi-Crescenzi, O

Biffoni, M

机构：

[1] Ist Ric Cesare Serono SpA, I-00040 Ardea, RM, Italy

[2] Univ Mainz, Dept Med, D-55101 Mainz, Germany

来源：

CYTOKINE | 2000年 / 12卷 / 09期

关键词：

CD34+; cord blood; erythropoiesis; interleukin; 6; stem cell factor;

D O I：

10.1006/cyto.2000.0723

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

H-IL-6 is a hybrid protein constructed to contain IL-6 and its soluble receptor linked by a flexible peptide chain. Here we show that H-IL-6 strongly enhances proliferation of human CD34(+) cells in serum-free liquid culture, and that the majority of the cells generated belong to the erythroid lineage, being positive for the marker Glycophorin A. Conversely, H-IL-6 does not increase the number of myeloid, CD13-positive cells. Comparable effects are observed on progenitors from cord blood and adult peripheral blood. Therefore, H-IL-6 triggers an erythroid-inducing signal in haematopoietic progenitor cells, independently from erythropoietin (EPO). (C) 2000 Academic Press.

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页码：1395 / 1399

页数：5

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