Direct Culture-Independent Strain Typing of Burkholderia cepacia Complex in Sputum Samples from Patients with Cystic Fibrosis

被引：13

作者：

Drevinek, Pavel ^{[1
]}

Vosahlikova, Sarka ^{[1
]}

Dedeckova, Klara ^{[1
]}

Cinek, Ondrej ^{[1
]}

Mahenthiralingam, Eshwar ^{[2
]}

机构：

[1] Charles Univ Prague, Dept Pediat, Sch Med 2, Prague 15006, Czech Republic

[2] Cardiff Univ, Cardiff Sch Biosci, Cardiff, S Glam, Wales

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 05期

关键词：

SP-NOV; MULTIVORANS; IDENTIFICATION; CENOCEPACIA;

D O I：

10.1128/JCM.02359-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.

引用

页码：1888 / 1891

页数：4

共 15 条

[1] Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex [J].

Baldwin, A ;

Mahenthiralingam, E ;

Thickett, KM ;

Honeybourne, D ;

Maiden, MCJ ;

Govan, JR ;

Speert, DP ;

LiPuma, JJ ;

Vandamme, P ;

Dowson, CG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (09) :4665-4673

[2] Burkholderia cepacia complex in cystic fibrosis:: Frequency of strain replacement during chronic infection [J].

Bernhardt, SA ;

Spilker, T ;

Coffey, T ;

LiPuma, JJ .

CLINICAL INFECTIOUS DISEASES, 2003, 37 (06) :780-785

[3] RecA gene sequence and Multilocus Sequence Typing for species-level resolution of Burkholderia cepacia complex isolates [J].

Cesarini, S. ;

Bevivino, A. ;

Tabacchioni, S. ;

Chiarini, L. ;

Dalmastri, C. .

LETTERS IN APPLIED MICROBIOLOGY, 2009, 49 (05) :580-588

[4] Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA [J].

Drevínek, P ;

Hrbácková, H ;

Cinek, O ;

Bartosová, J ;

Nyc, O ;

Nemec, A ;

Pohunek, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (09) :3485-3488

[5] Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia [J].

Drevinek, Pavel ;

Baldwin, Adam ;

Dowson, Christopher G. ;

Mahenthiralingam, Eshwar .

BMC MICROBIOLOGY, 2008, 8 (1)

[6] Burkholderia cenocepacia and Burkholderia multivorans:: influence on survival in cystic fibrosis [J].

Jones, AM ;

Dodd, ME ;

Govan, JRW ;

Barcus, V ;

Doherty, CJ ;

Morris, J ;

Webb, AK .

THORAX, 2004, 59 (11) :948-951

[7] DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III [J].

Mahenthiralingam, E ;

Bischof, J ;

Byrne, SK ;

Radomski, C ;

Davies, JE ;

Av-Gay, Y ;

Vandamme, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) :3165-3173

[8] The multifarious, multireplicon Burkholderia cepacia complex [J].

Mahenthiralingam, E ;

Urban, TA ;

Goldberg, JB .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (02) :144-156

[9] Multilocus Sequence Typing Breathes Life into a Microbial Metagenome [J].

Mahenthiralingam, Eshwar ;

Baldwin, Adam ;

Drevinek, Pavel ;

Vanlaere, Elke ;

Vandamme, Peter ;

LiPuma, John J. ;

Dowson, Chris G. .

PLOS ONE, 2006, 1 (01)

[10] Improved molecular detection of Burkholderia cepacia genomovar III and Burkholderia multivorans directly from sputum of patients with cystic fibrosis [J].

Moore, JE ;

Xu, JR ;

Millar, BC ;

Crowe, M ;

Elborn, JS .

JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 49 (02) :183-191

← 1 2 →