High-throughput screening of antibiotic-resistant bacteria in picodroplets

被引：90

作者：

Liu, X. ^{[1
]}

Painter, R. E. ^{[2
]}

Enesa, K. ^{[1
]}

Holmes, D. ^{[1
]}

Whyte, G. ^{[3
]}

Garlisi, C. G. ^{[2
]}

Monsma, F. J., Jr. ^{[2
]}

Rehak, M. ^{[1
]}

Craig, F. F. ^{[1
]}

Smith, C. A. ^{[1
]}

机构：

[1] Sphere Fluid Ltd, Jonas Webb Bldg,Babraham Res Campus, Cambridge CB22 3AT, England

[2] Merck & Co Inc, Merck Res Labs, 2015 Galloping Hill Rd,K15, Kenilworth, NJ 07033 USA

[3] Heriot Watt Univ, Inst Biol Chem Biophys & Bioengn, Sch Engn & Phys Sci, Edinburgh EH14 4AS, Midlothian, Scotland

来源：

LAB ON A CHIP | 2016年 / 16卷 / 09期

关键词：

SUSCEPTIBILITY;

D O I：

10.1039/c6lc00180g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The prevalence of clinically-relevant bacterial strains resistant to current antibiotic therapies is increasing and has been recognized as a major health threat. For example, multidrug-resistant tuberculosis and methicillin-resistant Staphylococcus aureus are of global concern. Novel methodologies are needed to identify new targets or novel compounds unaffected by pre-existing resistance mechanisms. Recently, water-in-oil picodroplets have been used as an alternative to conventional high-throughput methods, especially for phenotypic screening. Here we demonstrate a novel microfluidic-based picodroplet platform which enables high-throughput assessment and isolation of antibiotic-resistant bacteria in a label-free manner. As a proof-of-concept, the system was used to isolate fusidic acid-resistant mutants and estimate the frequency of resistance among a population of Escherichia coli (strain HS151). This approach can be used for rapid screening of rare antibiotic-resistant mutants to help identify novel compound/target pairs.

引用

页码：1636 / 1643

页数：8

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