Gold Nanocluster Containing Polymeric Microcapsules for Intracellular Ratiometric Fluorescence Biosensing

被引:35
作者
Biswas, Aniket [1 ]
Banerjee, Swayoma [2 ]
Gart, Elena V. [3 ]
Nagaraja, Ashvin T. [1 ]
McShane, Michael J. [1 ,4 ]
机构
[1] Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
[3] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX 77843 USA
[4] Texas A&M Univ, Dept Mat Sci & Engn, College Stn, TX 77843 USA
基金
美国国家科学基金会;
关键词
PROTEIN-DIRECTED SYNTHESIS; HYDROGEN-PEROXIDE; CANCER-CELLS; H2O2; PROBES; LUMINESCENCE; CARRIERS; SENSORS;
D O I
10.1021/acsomega.7b00199
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A new approach to sensing and imaging hydrogen peroxide (H2O2) was developed using microcapsule-based dual-emission ratiometric luminescent biosensors. Bovine serum albumin-capped gold nanoclusters (BSA-AuNCs) sensitive to H2O2 were coencapsulated with insensitive FluoSpheres (FSs) within polymeric capsules fabricated via the layer-by-layer method. Under single-wavelength excitation, the microcapsule-based biosensors exhibited emission bands at similar to 516 and similar to 682 nm resulting from the FSs and BSA-AuNCs, respectively. The polyelectrolyte multilayers lining the microcapsules were effective in protecting BSA-AuNCs from the degradation catalyzed by proteases (chymotrypsin, trypsin, papain, and proteinase K) and subsequent luminescent quenching, overcoming a key limitation of prior BSA-AuNC-based sensing systems. The luminescent response of the sensors was also found to be independent of local changes in pH (5-9). Quenching of the AuNCs in the presence of H2O2 enabled the spectroscopic quantification and imaging of changes in H2O2 concentration from 0 to 1 mM. The microcapsule sensors were easily phagocytized by murine macrophage cells (RAW 264.7), were effective as intracellular H2O2 imaging probes, and were successfully used to detect local release of H2O2 in response to an external chemical stimulus.
引用
收藏
页码:2499 / 2506
页数:8
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