FcLDP1, a Gene Encoding a Late Embryogenesis Abundant (LEA) Domain Protein, Responds to Brassinosteroids and Abscisic Acid during the Development of Fruits in Fragaria chiloensis

被引:8
作者
Espinoza, Analia [1 ]
Contreras, Rodrigo [2 ,3 ]
Zuniga, GustavoE. [2 ,3 ]
Herrera, Raul [4 ]
Alejandra Moya-Leon, Maria [4 ]
Norambuena, Lorena [1 ]
Handford, Michael [1 ]
机构
[1] Univ Chile, Fac Ciencias, Ctr Biol Mol Vegetal, Dept Biol, Santiago, Chile
[2] Univ Santiago Chile, Fac Quim & Biol, Santiago, Chile
[3] Univ Santiago Chile, Ctr Desarrollo Nanociencia & Nanotecnol, Santiago, Chile
[4] Univ Talca, Inst Ciencias Biol, Lab Fisiol Vegetaly & Genet Mol, Talca, Chile
关键词
abscisic acid; brassinosteroids; Fragaria chiloensis; fruit development; LEA domain protein; non-climacteric; TRANSCRIPTION FACTORS; SOFTENING RATE; STRAWBERRY; ARABIDOPSIS; EXPRESSION; IDENTIFICATION; BIOSYNTHESIS; SUPPRESSION; METABOLISM; DISSECTION;
D O I
10.3389/fpls.2016.00788
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
White Chilean strawberries (Fragaria chiloensis) are non-climacteric fruits, with an exotic color and aroma. In order to discover genes involved in the development of these fruits, we identified a fragment of a gene encoding a late embryogenesis abundant domain protein, FcLDP1, that was expressed in early stages of fruit development, particularly in receptacles. Hormones play key roles in regulating the development of non-climacteric fruits. We show that the brassinosteroid content of the white strawberry varies during development. Additionally, FcLDP1 as well as the closest ortholog in the woodland strawberry, F. vesca (FvLDP1) possess multiple brassinosteroid, as well as abscisic acid (ABA) response motifs in the promoter region, consistent with the response of transiently expressed FcLDP1 promoter-GFP fusions to these hormones, and the rise in FcLDP1 transcript levels in white strawberry fruits treated with brassinosteroids or ABA. These findings suggest that both hormones regulate FcLDP1 expression during the development of white strawberries.
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页数:11
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