Tuftsin-Phosphorylcholine Maintains Normal Gut Microbiota in Collagen Induced Arthritic Mice

被引:28
作者
Ben-Amram, Hila [1 ]
Bashi, Tomer [2 ]
Werbner, Nir [1 ]
Neuman, Hadar [1 ]
Fridkin, Mati [3 ]
Blank, Miri [2 ]
Shoenfeld, Yehuda [2 ]
Koren, Omry [1 ]
机构
[1] Bar Ilan Univ, Fac Med, Safed, Israel
[2] Tel Aviv Univ, Sackler Fac Med, Sheba Med Ctr, Zabludowicz Ctr Autoimmune Dis, Tel Aviv, Israel
[3] Weizmann Inst Sci, Dept Organ Chem, Rehovot, Israel
关键词
tuftsin-phosphorylcholine; collagen-induced arthritis; rheumatoid arthritis; helminths; microbiome; RHEUMATOID-ARTHRITIS;
D O I
10.3389/fmicb.2017.01222
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rheumatoid arthritis (RA) is characterized by chronic autoinflammation of the joints, with a prevalence of about 1% in Western populations. Evidence in recent years has linked RA to changes in the gut microbiota (dysbiosis). Interestingly, helminths have been shown to have therapeutic activity in RA. Specifically, a glycoprotein containing phosphorylcholine (PC) extracted from helminths was found to have immunomodulatory activity. We have previously developed a novel chimeric compound composed of tuftsin-PC (TPC) that attenuates the joint destruction in mice with collagen-induced arthritis (CIA). Here, we address the interrelationship between TPC immunomodulatory activity and the gut microbiota in CIA mice. Preventive therapy with TPC in mice with arthritis maintained a physiological arthritis score as well as a steady gut microbial environment, similar to that of healthy controls, in contrast to CIA mice with severe disease. The microbial composition differed significantly between healthy and phosphate-buffered saline-treated CIA mice, enabling classifying test samples by machine learning based on levels of a small number of bacterial species. Using these bacterial biomarkers, all TPC-treated CIA mice were classified as healthy. Thus, we describe a clear correlation between TPC treatment, healthy gut microbial communities, and prevention of arthritis. This is the first study to demonstrate the immunomodulatory effect of helminth derivatives in autoimmune diseases and the link to gut microbiota.
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页数:7
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