Antiviral Activity of Cassia Alata and Cosmos Caudatus Leaf Extract to Dengue Virus Serotype-2 New Guinea C Strain in Huh7-it1 Cell

被引：0

作者：

Prasetya, Septian Ika ^{[1
]}

Tanujaya, Adryan ^{[1
]}

Ratnasari, Shierly ^{[1
]}

Desti, Hidayati ^{[3
]}

Louisa, Melva ^{[3
]}

Angelina, Marisha ^{[4
]}

Sudiro, Tjahjani Mirawati ^{[2
]}

Dewi, Beti Ernawati ^{[2
]}

机构：

[1] Univ Indonesia, Med Fac, Jakarta, Indonesia

[2] Univ Indonesia, Med Fac, Dept Microbiol, Cipto Mangunkusumo Hosp, Jalan Pegangsaan Timur 16, Jakarta, Indonesia

[3] Univ Indonesia, Med Fac, Dept Pharmacol, Cipto Mangunkusumo Hosp, Jalan Salemba Raya 6, Jakarta, Indonesia

[4] Indonesian Inst Sci, Res Ctr Chem, Kawasan Puspitek Serpong 15416, Indonesia

来源：

ADVANCED SCIENCE LETTERS | 2018年 / 24卷 / 09期

关键词：

Antiviral Activity; Cassia Alata; Cosmos Caudatus; Dengue; Half-Inhibitory Concentration; Half-Cytotoxic Concentration;

D O I：

10.1166/asl.2018.12725

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Introduction: Dengue virus infection is still a major health problem in Indonesia where anually thousands of people underwent dengue haemorrhagic fever and hundreds of people died. Dengue antivirals is still unavailable and various natural extracts were tested for its potential as an active substance for it. The objective of this study is to evaluate antiviral activity of leaf extract of Cassia alata and Cosmos caudatus against dengue virus serotype-2 (DENV-2) in vitro. Method: We used Huh7-it-1 cell line and DENV2 strain New Guinea C. The varying concentration of each separate leaf extract were 10, 20, 40, 80, 160, and 320 mu g/mL. Extract's inhibitory capability and toxicity were evaluated by focus assay and MTT assay, respectively. Focus assay measured the decrement of virus titer after DENV2 were treated with various concentration of the extracts and then the mean percentage of inhibition of each concentration were plotted against the respective concentration to generate concentration-mean infectivity percentage curves. Meanwhile, MTT assay was performed by exposing various concentration of the extracts to the cell culture and then quantified cell's viability by absorbance reading to obtain concentration-mean percentage of cell viability curve. Nonlinear regression analysis was performed upon concentration-effect curves of infectivity and viability to determine half-inhibitory concentration (IC50) and half-cytotoxic concentration (CC50), respectively. Selectivity index ( SI) as a measure of antiviral activity is a ratio between CC50 and IC50. Result: The Cassia alata leaf extract had the IC50, CC50 and SI of <10 mu g/mL, 323.45 mu g/mL, and >32.3 respectively. Meanwhile, the IC50, CC50, and SI of Cosmos caudatus leaf extract were 12.2 mu g/mL, 187.1 mu g/mL and 15.4 respectively. Conclusion: Given their high selectivity indices, Cassia alata and Cosmos caudatus leaf extract showed potent antiviral activity against DENV2. Further studies are required to to identify active compound of Cassia alata and Cosmos caudatus leaf that have potency as antiviral for DENV and their mechanism of inhibition.

引用

页码：6388 / 6392

页数：5