Involvement of p38 MAPK pathway in benzo(a)pyrene-induced human hepatoma cell migration and invasion

被引：6
作者：
Wang, Yadong [1 ]
Shi, Li [2 ]
Li, Jiangmin [1 ]
Li, Li [1 ]
Wang, Haiyu [1 ]
Yang, Haiyan [2 ]
机构：
[1] Henan Ctr Dis Control & Prevent, Dept Toxicol, 105 South Nongye Rd, Zhengzhou 450016, Henan, Peoples R China
[2] Zhengzhou Univ, Sch Publ Hlth, Dept Epidemiol, 100 Sci Ave, Zhengzhou 450001, Henan, Peoples R China
来源：
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 2019年 / 26卷 / 35期
基金：
中国国家自然科学基金;
关键词：
Benzo(a)pyrene; Migration; Invasion; p38; MAPK; MMP9; HepG2; cells; NF-KAPPA-B; HEPATOCELLULAR-CARCINOMA; PRIMARY HEPATOCYTES; PROMOTES MIGRATION; CANCER; METASTASIS; MATRIX-METALLOPROTEINASE-9; CARCINOGENESIS; PROLIFERATION; ANGIOGENESIS;
D O I：
10.1007/s11356-019-06733-3
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The objective of this study was to investigate the potential role of p38 mitogen-activated protein kinases (MAPK) in benzo(a)pyrene (BaP)-induced hepatoma cell migration and invasion. Western blot assay was applied to detect the expression of proteins. qRT-PCR assay was used to measure the expression of mRNA. Wound healing assay and Transwell invasion assay were performed to evaluate cell migratory ability and cell invasive ability, respectively. Our data showed that BaP exposure increased the expression of p-p38 protein in human hepatoma HepG2 cells. Exposure to BaP facilitated HepG2 cell migration and invasion, which could be blocked by p38 MAPK inhibitors. In addition, BaP exposure induced upregulation of MMP9 mRNA expression, which was modulated by p-p38. In conclusion, p38 MAPK pathway was involved in BaP-induced hepatoma cell migration and invasion.
引用
收藏
页码：35838 / 35845
页数：8
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