Cytotoxic, genotoxic, and carcinogenic effects of acrylamide on human lung cells

被引:20
作者
Yedier, Seval Kontas [1 ]
Sekeroglu, Zlal Atli [1 ,3 ]
Sekeroglu, Vedat [1 ]
Aydin, Birsen [2 ]
机构
[1] Ordu Univ, Fac Sci & Letters, Dept Mol Biol & Genet, Ordu, Turkey
[2] Amasya Univ, Fac Sci & Letters, Dept Biol, Amasya, Turkey
[3] Ordu Univ, Fac Sci & Letters, Dept Mol Biol & Genet, TR-52200 Ordu, Turkey
关键词
Acrylamide; DNA strand Breaks; Cell transformation; Human lung cells; PROSTATE-CANCER RISK; DIETARY ACRYLAMIDE; BREAST-CANCER; PROSPECTIVE COHORT; OXIDATIVE STRESS; POSTMENOPAUSAL WOMEN; HEMOGLOBIN ADDUCTS; OVARIAN-CANCER; URINARY LEVELS; DNA-DAMAGE;
D O I
10.1016/j.fct.2022.112852
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
While an association between acrylamide (AC) exposure and the risk of developing cancer has been shown in some studies, there are very limited data on the relationship between AC exposure and lung cancer risk. Thus, we investigated the cytotoxic, genotoxic, and carcinogenic effects of AC on human lung bronchial epithelial cell line (BEAS-2B cells). AC (5 and 10 mM) significantly decreased the cell viability for all treatment times. The comet assay results showed that AC (0.5, 1 and 5 mM) increased the DNA tail (%), tail moment and olive tail moment. By using immunofluorescence, we found that AC (0.5, 1 and 5 mM) induced the formation of both phosphorylated form of the histone H2 variant H2AX (gH2AX) and p53-binding protein 1 (53BP1) foci. AC-treated BEAS2B cells exhibited various morphological and cytoplasmic changes. The transformed cells can induce form foci and significantly increase the number of colonies in soft agar. We showed for the first time that AC could induce DNA strand breaks, cell transformation, and anchorage-independent growth in BEAS-2B cells. Therefore, AC exposure can induce carcinogenesis in lung cells and may be a risk for lung cancer formation. Further studies are necessary to make a possible risk assessment in humans.
引用
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页数:11
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