MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children

被引:29
作者
Huang, Feng [1 ,2 ]
Zhang, Junsong [2 ]
Yang, Diyuan [1 ]
Zhang, Yuelan [3 ]
Huang, Jinxiang [3 ]
Yuan, Yaochang [2 ]
Li, Xuefeng [3 ]
Lu, Gen [1 ]
机构
[1] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangzhou 510120, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Inst Human Virol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
[3] Guangzhou Med Univ, Guangdong Prov Key Lab Allergy & Clin Immunol, Sino French Hoffmann Inst,Sch Basic Med Sci, State Key Lab Resp Dis,Qingyuan Peoples Hosp, Guangzhou 511436, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
VIRUS; INHIBITION; UBE2V2; RNA;
D O I
10.1155/2018/2320640
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. MicroRNAs (miRNAs) are associated with viral replication and host immune response. Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. In our study, total RNA extracted from whole blood of adenovirus-infected pneumonia children and healthy controls were analyzed by small RNA deep sequencing. Expression profiles of whole blood microRNAs were altered and distinctly different in adenovirus-infected children. The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-milt-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. Potential host target genes were predicated and validated by qRT-PCR to study the impact of microRNAs on the host genes. Most of the target genes were involved in the MAPK signaling pathway and innate immune response. These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia.
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收藏
页数:11
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