Nitric oxide (NO) disrupts specific DNA binding of the transcription factor c-Myb in vitro

被引:43
作者
Brendeford, EM [1 ]
Andersson, KB [1 ]
Gabrielsen, OS [1 ]
机构
[1] Univ Oslo, Dept Biochem, N-0316 Oslo 3, Norway
关键词
Myb; DNA binding; avian myeloblastosis virus; nitric oxide; redox;
D O I
10.1016/S0014-5793(98)00196-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In an attempt to elucidate signal transduction pathways which mag modulate DNA binding of the transcription factor c-Myb, we investigated whether c-Myb could be a target for the signaling molecule nitric oxide (NO) in vitro, NO-generating agents severely inhibited specific DNA binding of the c-Myb minimal DNA-binding domain R2R3., This inhibition was readily reversible upon treatment with excess DTT. A redox-sensitive cysteine (C130) was required for this NO sensitivity. Moreover, a DNA-binding domain carrying two of the avian myeloblastosis virus (AMV)-specific mutations (L106H, V117D) appeared to be less sensitive to S-nitrosylation than the wild-type c-Myb. This difference in NO sensitivity may influence the regulation of wild type versus AMV v-Myb protein function. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:52 / 56
页数:5
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