EFFECTS OF LNCRNA-UFC1 ON PROLIFERATION ACTIVITY, APOPTOSIS RATE AND GROWTH OF CHONDROCYTES IN OSTEOARTHRITIS

Objective: To investigate the effects of LncRNA-UFCI on proliferation activity, apoptosis, rate and growth of chondrocytes in osteoarthritis (OA). Methods: Fifty- one cases of chondrocytes in the joint and normal chondrocytes of OA patients adtitted to our hosptal from march 2016 to November 2017 were slected. The chondrocytes were divided into three groups (A, B and C) after a stRNA transfection experiment,among which the A grup were conventional osteoarthritis chondrocytes, B group were osteoarthritis chondrocytes Methods: Fifty- one cases of chondrocytes in the joint and normal chondrocytes of OA patients adtitted to our hosptal from march 2016 to November 2017 were slected. The chondrocytes were divided into three groups (A, B and C) after a stRNA transfection experiment,among which the A grup were conventional osteoarthritis chondrocytes, B group were osteoarthritis chondrocytes co-cultured with empy plasmid and liposome, and the group were osteoarthritis co-cultured with UFCI-siRNA-liposome comples. lncRNA-UFCI expression and behavioural osteoarthritis of osteoarthritis in OA and nomal co-cultured with empy plasmid and liposome, and the group were osteoarthritis co-cultured with UFCI-siRNA-liposome comples. lncRNA-UFCI expression and behavioural osteoarthritis of Methods: Fifty- one cases of chondrocytes in the joint and normal chondrocytes of OA patients adtitted to our hosptal from march 2016 to November 2017 were slected. The chondrocytes were divided into three groups (A, B and C) after a stRNA transfection experiment,among which the A grup were conventional osteoarthritis chondrocytes, B group were osteoarthritis chondrocytes co-cultured with empy plasmid and liposome, and the group were osteoarthritis co-cultured with UFCI-siRNA-liposome comples. lncRNA-UFCI expression and behavioural osteoarthritis of Methods: Fifty- one cases of chondrocytes in the joint and normal chondrocytes of OA patients adtitted to our hosptal from march 2016 to November 2017 were slected. The chondrocytes were divided into three groups (A, B and C) after a stRNA transfection experiment,among which the A grup were conventional osteoarthritis chondrocytes, B group were osteoarthritis chondrocytes co-cultured with empy plasmid and liposome, and the group were osteoarthritis co-cultured with UFCI-siRNA-liposome comples. lncRNA-UFCI expression and behavioural osteoarthritis of chondrocytes in OA and nomal chondrocytes, and over- expression complex. lncRNA-UFCI expression and behavioural osteoarthritis of chondrocytes in OA were observed. Results: The RT-PCR Results showed tha the LncRNA-UFCI expression behavioural osteoarthritis of chondrocytes was significantly lower than thar in normal chondrocytes, and the difference was ststistically significsnt (P <0.05). the results of a CCK8 test showed thaat thr proliferation activity of osteoarthritic chondrocytes ( P<0.05). the result of flow cytometry Pressl rlevelo fenondrc rate of osteoarthitic chondroytes results of UFCI over-expression showed thst the UFCI expression of chondrocytes in C group was significantly higer than in the C group were markedly higer than thpose in the A grup (P <0.05), and the apoptosis rate of chondrocytes in the C group was significantly higer than in the A group (P <0.05). experitment results of UFCI silencing showed thaat UFCI expression of chondrocytes in C group was significantly higer than in the A group (P <0.05), and B group hadno UFCI expression. The grwoth rate and proliferation activity of in the C group was significantly higer than in the A group (P <0.05), whreas the apoptosis rate of in the C group was significantly higer than in the A group (P <0.05). Conclusion: lncRna-UFCI can promote the proliferation activity of osteoarthitic chondroytes, inhibit the apoptosis of osteoarthitic chondroytes and promote the growth of chondroytes.