Metagenomic approaches to exploit the biotechnological potential of the microbial consortia of marine sponges

被引:80
作者
Kennedy, Jonathan
Marchesi, Julian R.
Dobson, Alan D. W. [1 ]
机构
[1] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland
[2] Natl Univ Ireland Univ Coll Cork, Environm Res Inst, Cork, Ireland
[3] Natl Univ Ireland Univ Coll Cork, Alimentary Pharmabiot Ctr, Cork, Ireland
关键词
metagenomics; marine sponges; natural products;
D O I
10.1007/s00253-007-0875-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Natural products isolated from sponges are an important source of new biologically active compounds. However, the development of these compounds into drugs has been held back by the difficulties in achieving a sustainable supply of these often-complex molecules for pre-clinical and clinical development. Increasing evidence implicates microbial symbionts as the source of many of these biologically active compounds, but the vast majority of the sponge microbial community remain uncultured. Metagenomics offers a biotechnological solution to this supply problem. Metagenomes of sponge microbial communities have been shown to contain genes and gene clusters typical for the biosynthesis of biologically active natural products. Heterologous expression approaches have also led to the isolation of secondary metabolism gene clusters from uncultured microbial symbionts of marine invertebrates and from soil metagenomic libraries. Combining a metagenomic approach with heterologous expression holds much promise for the sustainable exploitation of the chemical diversity present in the sponge microbial community.
引用
收藏
页码:11 / 20
页数:10
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