The ldb1 mutant of Saccharomyces cerevisiae is defective in Pmr1p, the yeast secretory pathway/Golgi Ca2+/Mn2+-ATPase

被引:9
|
作者
Olivero, I [1 ]
Corbacho, I [1 ]
Hernández, LM [1 ]
机构
[1] Univ Extremadura, Dept Microbiol, E-06071 Badajoz, Spain
关键词
Saccharomyces cerevisiae; glycosylation; phosphorylation; golgi apparatus; LDB1; PMR1;
D O I
10.1016/S0378-1097(03)00002-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The LDB1 gene of Saccharomyces cerevisiae was identified by complementation of the ldb1 mutant phenotype with a genomic library. We found that the ldb1 defect is complemented by PMR1 which codes for the yeast secretory pathway/Golgi Ca2+/Mn2+-ATPase. Besides that, the analysis of a null mutation of the PMR1 gene revealed a phenotype identical to that of ldb1 mutant. Thus, LDB1 must be considered a synonym of PMR1. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:137 / 142
页数:6
相关论文
共 50 条
  • [31] Silencing the SPCA1 (Secretory Pathway Ca2+-ATPase Isoform 1) Impairs Ca2+ Homeostasis in the Golgi and Disturbs Neural Polarity
    Sepulveda, M. Rosario
    Vanoevelen, Jo
    Raeymaekers, Luc
    Mata, Ana M.
    Wuytack, Frank
    JOURNAL OF NEUROSCIENCE, 2009, 29 (39): : 12174 - 12182
  • [32] Elevated cytosolic free Ca2+ concentrations and massive Ca2+ accumulation within vacuoles, in yeast mutant lacking PMR1, a homolog of Ca2+-ATPase
    Halachmi, D
    Eilam, Y
    FEBS LETTERS, 1996, 392 (02) : 194 - 200
  • [33] Molecular cloning of YlPMR1, a S-cerevisiae PMR1 homologue encoding a novel P-type secretory pathway CA2+-ATPase, in the yeast Yarrowia lipolytica
    Park, CS
    Kim, YY
    Crispino, C
    Chang, CC
    Ryu, DDY
    GENE, 1998, 206 (01) : 107 - 116
  • [34] PMR1/SPCA Ca2+ pumps and the role of the Golgi apparatus as a Ca2+ store
    Frank Wuytack
    Luc Raeymaekers
    Ludwig Missiaen
    Pflügers Archiv, 2003, 446 : 148 - 153
  • [35] PMR1/SPCA Ca2+ pumps and the role of the Golgi apparatus as a Ca2+ store
    Wuytack, F
    Raeymaekers, L
    Missiaen, L
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2003, 446 (02): : 148 - 153
  • [36] Deletion of the Golgi Ca2+-ATPase PMR1 gene potentiates antifungal effects of dodecanol that depend on intracellular Ca2+ accumulation in budding yeast
    Oyama, Masahiro
    Tamaki, Hiroyuki
    Yamaguchi, Yoshihiro
    Ogita, Akira
    Tanaka, Toshio
    Fujita, Ken-ichi
    FEMS YEAST RESEARCH, 2020, 20 (01)
  • [37] Mechanisms of synergy of autoantibodies to M3 muscarinic acetylcholine receptor and secretory pathway Ca2+/Mn2+-ATPase isoform 1 in patients with non-desmoglein pemphigus vulgaris
    Chernyaysky, Alex
    Patel, Krupa G.
    Grando, Sergei A.
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2020, 80
  • [38] SPCA2 couples Ca2+ influx via Orai1 to Ca2+ uptake into the Golgi/secretory pathway
    Smaardijk, Susanne
    Chen, Jialin
    Wuytack, Frank
    Vangheluwe, Peter
    TISSUE & CELL, 2017, 49 (02): : 141 - 149
  • [39] Extracellular Ca2+ sensing contributes to excess Ca2+ accumulation and vacuolar fragmentation in a pmr1Δ mutant of S-cerevisiae
    Kellermayer, R
    Aiello, DP
    Miseta, A
    Bedwell, DM
    JOURNAL OF CELL SCIENCE, 2003, 116 (08) : 1637 - 1646
  • [40] The Secretory Pathway Ca2+/Mn2+-Atpase SPCA2 Regulates Mn2+-Dependent Cell Cycle Progression in 3D Culture of Colon Cancer Cells
    Jenkins, James
    Dmitriev, Ruslan
    Papkovsky, Dmitri
    BIOPHYSICAL JOURNAL, 2016, 110 (03) : 336A - 336A