Cytogenetics of three breeds of river buffalo (Bubalus bubalis L.), with evidence of a fragile site on the X chromosome

被引:10
作者
Pires, RML
Reichert, RH
Kasahara, S
机构
[1] Inst Zootecnia, Dept Anim Reprod, BR-13460000 Nova Odessa, SP, Brazil
[2] Univ Estadual Paulista, Dept Biol, Sao Paulo, Brazil
关键词
fragile site; Bubalus bubalis L; chromosome banding; buffal cytogenetics; X chromosome;
D O I
10.1016/S0093-691X(98)00004-1
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The cytogenetic study of 182 river buffalo (Bubalus bubalis L., 2n=50) of Murrah, Mediterranean and Jaffarabadi breeds, from the State of Sao Paulo, was carried out to characterize their chromosomes and to detect possible chromosomal abnormalities. The karyotypes were indistinguishable with conventional staining as well as with C and replication R banding techniques. In about 44% of the sample (8 males and 72 females), an X marker chromosome due to a fragile site was shown. The frequency of metaphases expressing the fragility site on the X was highly variable, from 2.86 to 41.03%. In females, the fragile site, rarely appeared on both X chromosomes. Most of the metaphases showed only 1 marker chromosome. In R-banded metaphases using 5-bromodeoxyuridine (BrdU) treatment, it corresponded in general to the late replicating X chromosome. No correlation between the X fragile site and altered phenotype was found. Structural and numerical chromosome rearrangements were ruled out in the present sample of buffalo. (C) 1998 by Elsevier Science Inc.
引用
收藏
页码:529 / 538
页数:10
相关论文
共 39 条
[1]  
Balakrishnan C. R., 1988, Buffalo production and health. A compendium of latest research information based on Indian studies., P16
[2]  
Balakrishnan C. R., 1984, Buffalo Bulletin, V3, P13
[3]  
CRIBIU EP, 1978, ANN GENET SEL ANIM, V10, P271, DOI 10.1186/1297-9686-10-2-271
[4]   FOLIC-ACID SENSITIVE FRAGILE SITES ARE NOT LIMITED TO THE HUMAN KARYOTYPE - DEMONSTRATION OF NONRANDOM GAPS AND BREAKS IN THE PERSIAN VOLE ELLOBIUS-LUTESCENS-TH INDUCIBLE BY METHOTREXATE, FLUORODEOXYURIDINE, AND APHIDICOLIN [J].
DIALALI, M ;
BARBI, G ;
STEINBACH, P .
HUMAN GENETICS, 1985, 70 (02) :183-185
[5]  
DIBERARDINO D, 1984, GENET SEL EVOL, V16, P249, DOI 10.1186/1297-9686-16-3-249
[6]  
DUTRILLAUX B., 1981, PRATIQUE ANAL CHROMO
[7]  
GENEST P, 1979, CAN J COMP MED, V43, P110
[8]   A FRAGILE SITE IN THE X-CHROMOSOME OF THE REINDEER (RANGIFER-TARANDUS-L) [J].
GRIPENBERG, U ;
HUUHTANEN, S ;
WESSMAN, M ;
NIEMINEN, M .
GENETICS SELECTION EVOLUTION, 1991, 23 :S135-S139
[9]  
GUPTA P, 1978, NUCLEUS CALCUTTA, V21, P90
[10]  
HONDT HA, 1971, Z TIERZUCHT ZUCHTGSB, V88, P64