Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes

被引：41

作者：

Machan, Radek ^{[1
]}

Hof, Martin ^{[1
]}

机构：

[1] ASCR, J Heyrovsky Inst Phys Chem, Vvi, Prague 18223, Czech Republic

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 02期

关键词：

lateral diffusion; fluorescence fluctuation spectroscopy; confocal microscopy; biomembranes; supported lipid bilayers; giant unilamellar vesicles; IMAGE CORRELATION SPECTROSCOPY; GIANT UNILAMELLAR VESICLES; INTERNAL-REFLECTION FLUORESCENCE; SUPPORTED PHOSPHOLIPID-BILAYERS; LASER-SCANNING MICROSCOPE; SINGLE-PARTICLE TRACKING; ATOMIC-FORCE MICROSCOPY; OIL-WATER INTERFACE; MODE WAVE-GUIDES; LATERAL DIFFUSION;

D O I：

10.3390/ijms11020427

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We focus on diffusion measurements of lipids and proteins in planar lipid membranes and review the contributions of FCS to elucidating membrane dynamics and the factors influencing it, such as membrane composition, ionic strength, presence of membrane proteins or frictional coupling with solid support.

引用

页码：427 / 457

页数：31

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