Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes

被引：41

作者：

Machan, Radek ^{[1
]}

Hof, Martin ^{[1
]}

机构：

[1] ASCR, J Heyrovsky Inst Phys Chem, Vvi, Prague 18223, Czech Republic

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 02期

关键词：

lateral diffusion; fluorescence fluctuation spectroscopy; confocal microscopy; biomembranes; supported lipid bilayers; giant unilamellar vesicles; IMAGE CORRELATION SPECTROSCOPY; GIANT UNILAMELLAR VESICLES; INTERNAL-REFLECTION FLUORESCENCE; SUPPORTED PHOSPHOLIPID-BILAYERS; LASER-SCANNING MICROSCOPE; SINGLE-PARTICLE TRACKING; ATOMIC-FORCE MICROSCOPY; OIL-WATER INTERFACE; MODE WAVE-GUIDES; LATERAL DIFFUSION;

D O I：

10.3390/ijms11020427

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We focus on diffusion measurements of lipids and proteins in planar lipid membranes and review the contributions of FCS to elucidating membrane dynamics and the factors influencing it, such as membrane composition, ionic strength, presence of membrane proteins or frictional coupling with solid support.

引用

页码：427 / 457

页数：31

共 210 条

[1] LATERAL DIFFUSION IN THE LIQUID-PHASES OF DIMYRISTOYLPHOSPHATIDYLCHOLINE CHOLESTEROL LIPID BILAYERS - A FREE-VOLUME ANALYSIS [J].

ALMEIDA, PFF ;

VAZ, WLC ;

THOMPSON, TE .

BIOCHEMISTRY, 1992, 31 (29) :6739-6747

[2] Direct visualization of membrane leakage induced by the antibiotic peptides: Maculatin, citropin, and aurein [J].

Ambroggio, EE ;

Separovic, F ;

Bowie, JH ;

Fidelio, GD ;

Bagatolli, LA .

BIOPHYSICAL JOURNAL, 2005, 89 (03) :1874-1881

[3] LIPOSOME ELECTROFORMATION [J].

ANGELOVA, MI ;

DIMITROV, DS .

FARADAY DISCUSSIONS, 1986, 81 :303-+

[4] MOBILITY MEASUREMENT BY ANALYSIS OF FLUORESCENCE PHOTOBLEACHING RECOVERY KINETICS [J].

AXELROD, D ;

KOPPEL, DE ;

SCHLESSINGER, J ;

ELSON, E ;

WEBB, WW .

BIOPHYSICAL JOURNAL, 1976, 16 (09) :1055-1069

[5] Fluorescence correlation spectroscopy relates rafts in model and native membranes [J].

Bacia, K ;

Scherfeld, D ;

Kahya, N ;

Schwille, P .

BIOPHYSICAL JOURNAL, 2004, 87 (02) :1034-1043

[6] Giant phospholipid vesicles: comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study [J].

Bagatolli, LA ;

Parasassi, T ;

Gratton, E .

CHEMISTRY AND PHYSICS OF LIPIDS, 2000, 105 (02) :135-147

[7] SPECULAR REFLECTION OF NEUTRONS AT PHOSPHOLIPID MONOLAYERS - CHANGES OF MONOLAYER STRUCTURE AND HEADGROUP HYDRATION AT THE TRANSITION FROM THE EXPANDED TO THE CONDENSED PHASE STATE [J].

BAYERL, TM ;

THOMAS, RK ;

PENFOLD, J ;

RENNIE, A ;

SACKMANN, E .

BIOPHYSICAL JOURNAL, 1990, 57 (05) :1095-1098

[8] The structure, dynamics and orientation of antimicrobial peptides in membranes by multidimensional solid-state NMR spectroscopy [J].

Bechinger, B .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1462 (1-2) :157-183

[9] How to determine diffusion coefficients in planar phospholipid systems by confocal fluorescence correlation spectroscopy [J].

Benda, A ;

Benes, M ;

Marecek, V ;

Lhotsky, A ;

Hermens, WT ;

Hof, M .

LANGMUIR, 2003, 19 (10) :4120-4126

[10] Fluorescence lifetime correlation spectroscopy combined with lifetime tuning:: New perspectives in supported phospholipid bilayer research [J].

Benda, Ales ;

Fagul'ova, Veronika ;

Deyneka, Alexander ;

Enderlein, Joerg ;

Hof, Martin .

LANGMUIR, 2006, 22 (23) :9580-9585

← 1 2 3 4 5 6 7 8 9 10 →