The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species

被引：7

作者：

Tomita, Shunsuke ^{[1
]}

Yokoyama, Saki ^{[2
]}

Kurita, Ryoji ^{[1
]}

Niwa, Osamu ^{[1
]}

Yosmmoirot, Keitaro ^{[2
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, 1-1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan

[2] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan

来源：

ANALYTICAL SCIENCES | 2016年 / 32卷 / 02期

关键词：

Biosensors; discriminant analysis; enzymes; block-copolymers; serum; PATTERN-RECOGNITION ANALYSIS; PROTEINS; IDENTIFICATION;

D O I：

10.2116/analsci.32.237

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A cross-reactive sensor array consisting of polyion complexes (PICs) between anionic enzymes and poly(ethylene glycol)-modified (PEGylated) polyamines has been used to identify the source of mammalian sera. Although the catalytic activity of enzymes was inhibited by PIC formation with PEGylated polyamines, the subsequent addition of sera caused enzyme releases from PICs through competitive interactions between PICs and serum proteins, generating unique response patterns of changes in the enzyme activity. Linear discriminant analysis of the obtained patterns enabled the discrimination of five sera from different mammalian sources.

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收藏

页码：237 / 240

页数：4

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