Progesterone increases mRNA levels of pituitary adenylate cyclase-activating polypeptide (PACAP) and type IPACAP receptor (PAC1) in the rat hypothalamus

被引:33
作者
Ha, CM
Kang, JH
Choi, EJ
Kim, MS
Park, JW
Kim, Y
Choi, WS
Chun, SY
Kwon, HB
Lee, BJ [1 ]
机构
[1] Univ Ulsan, Dept Biol Sci, Ulsan 680749, South Korea
[2] Univ Ulsan, Coll Med, Dept Neurosurg, Ulsan 680749, South Korea
[3] Gyeongsang Natl Univ, Coll Med, Dept Anat, Chinju 682060, South Korea
[4] Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea
来源
MOLECULAR BRAIN RESEARCH | 2000年 / 78卷 / 1-2期
关键词
PACAP; PACAP receptor type I (PAC(1)); progesterone; antisense PAC(1) oligodeoxynucleotide; LHRH mRNA;
D O I
10.1016/S0169-328X(00)00070-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Pituitary adenylate cyclase-activating polypeptide (PACAP) regulates pituitary hormone biosynthesis and secretion through its cognate receptors. PACAP also plays an important role in the regulation of ovarian steroid biosynthesis. If so, there might be a feedback regulation of hypothalamic PACAP synthesis by the pituitary and by ovarian steroids. In the present study, we used RNase protection assays to determine changes in mRNA levels of PACAP and type I PACAP receptor (PAC,) under the conditions of ovariectomy and replacement with ovarian steroids. Progesterone (P) alone or in combination with estradiol (E) induced significant increases in PACAP mRNA level in the medial basal hypothalamus (MBH) and PAC, mRNA levels in MBH and the preoptic area (POA). This finding suggests that feedback regulation takes place between the ovary and hypothalamic PACAP neurons. P is known to be a major regulatory feedback factor for hypothalamic luteinizing hormone-releasing hormone (LHRH) neurons, but P receptor is not present in these neurons. Therefore, we examined a possible involvement of PACAP in the feedback regulatory pathway of P to LHRH neurons. After an antisense PAC, oligodeoxynucleotide (ODN) was i.c.v.-injected into the third ventricle of E and P-treated rats, LHRH mRNA levels were determined. The ODN markedly decreased the P-induced increase in the LHRH mRNA level. Taken together, the present data suggest that PACAP may play a role as a mediator in the regulation of LHRH synthetic machinery by stimulatory feedback of P. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:59 / 68
页数:10
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