IGF2BP2 promotes colorectal cancer cell proliferation and survival through interfering with RAF-1 degradation by miR-195

被引：86

作者：

Ye, Song ^{[1
]}

Song, Wei ^{[2
]}

Xu, Xiaogang ^{[3
]}

Zhao, Xinyi ^{[2
]}

Yang, Liu ^{[3
,4
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, Hangzhou 310003, Zhejiang, Peoples R China

[2] Minist Publ Hlth, Key Lab Combined Multiorgan Transplantat, Key Lab Organ Transplantat, Hangzhou, Zhejiang, Peoples R China

[3] Zhejiang Prov Peoples Hosp, Key Lab Tumor Mol Diag & Individualized Med Zheji, Hangzhou 310014, Zhejiang, Peoples R China

[4] Zhejiang Prov Peoples Hosp, Dept Gastroenterol & Pancreat Surg, Hangzhou 310014, Zhejiang, Peoples R China

来源：

FEBS LETTERS | 2016年 / 590卷 / 11期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

colorectal cancer; IGF2BP2; miR-195; RAF1; RNA-binding proteins; RNA-BINDING PROTEIN; MESSENGER-RNA; BREAST-CANCER; COLON-CANCER; LUNG-CANCER; EXPRESSION; IMP2; TRANSLATION; PROGRESSION; PATHWAYS;

D O I：

10.1002/1873-3468.12205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin-like growth factor 2 (IGF2) mRNA-binding protein 2 (IGF2BP2) is a post-transcriptional regulatory factor implicated in mRNA localization, stability, and translational control. However, the role of IGF2BP2 regulation in colorectal cancer (CRC) and its underlying mechanism remain elusive. In this study, we found that IGF2BP2 expression is markedly increased in CRC tissues. Notably, IGF2BP2 overexpression strikingly enhanced the proliferation and survival of CRC cells in vitro, whereas its shRNA-mediated silencing resulted in the opposite. Molecular function analyses revealed that IGF2BP2 regulates RAF1 expression through blocking its degradation by miR-195. These results identify IGF2BP2 as a post-transcriptional regulatory mRNA-binding factor that contributes to CRC carcinogenesis.

引用

页码：1641 / 1650

页数：10

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