A highly pathogenic porcine reproductive and respiratory syndrome virus candidate vaccine based on Japanese encephalitis virus replicon system

被引：3

作者：

Hu, Pingsheng ^{[1
]}

Chen, Xiaoming ^{[1
]}

Huang, Lihong ^{[1
]}

Liu, Shukai ^{[1
]}

Zang, Fuyu ^{[1
]}

Xing, Jinchao ^{[1
]}

Zhang, Youyue ^{[1
]}

Liang, Jiaqi ^{[1
]}

Zhang, Guihong ^{[1
]}

Liao, Ming ^{[1
,2
,3
]}

Qi, Wenbao ^{[1
,2
,3
]}

机构：

[1] South China Agr Univ, Coll Vet Med, Natl & Reg Joint Engn Lab Medicament Zoonoses Pre, Guangzhou, Guangdong, Peoples R China

[2] Minist Agr, Key Lab Zoonoses, Key Lab Anim Vaccine Dev, Guangzhou, Guangdong, Peoples R China

[3] Minist Agr, Key Lab Zoonoses Prevent & Control Guangdong Prov, Guangzhou, Guangdong, Peoples R China

来源：

PEERJ | 2017年 / 5卷

基金：

中国国家自然科学基金;

关键词：

JEV replicon; HP-PRRSV; Vaccine; LIVE VACCINE; PRRS MLV; IN-VITRO; CHINA; GENE; CONSTRUCTION; REPLICATION; CHALLENGES; PROTECTION; EMERGENCE;

D O I：

10.7717/peerj.3514

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the swine industry, porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease which causes heavy economic losses worldwide. Effective prevention and disease control is an important issue. In this study, we described the construction of a Japanese encephalitis virus (JEV) DNA-based replicon with a cytomegalovirus (CMV) promoter based on the genome of Japanese encephalitis live vaccine virus SA14-14-2, which is capable of offering a potentially novel way to develop and produce vaccines against a major pathogen of global health. This JEV DNA-based replicon contains a large deletion in the structural genes (C-prM-E). A PRRSV GP5/M was inserted into the deletion position of JEV DNA based replicons to develop a chimeric replicon vaccine candidate for PRRSV The results immune that BALB/c mice models with the replicon vaccines pJEV-REP-G-2A-M-IRES and pJEV-REP-G-2A-M stimulated antibody responses and induced cellular response. Analysis of ELSA data showed that vaccination with the replicon vaccine expressing GP5/M induced a better antibodies response than traditional DNA vaccines. Therefore, the results suggested that this ectopic expression system based. on DNA-based replicons may represent a useful molecular platform for various biological applications, and the DNA based replicons expressing GP5/M can be further developed into a novel, safe vaccine candidate for PRRS.

引用

页数：19

共 36 条

[1] Humoral and cellular immune response to RNA immunization with flavivirus Replicons derived from tick-borne encephalitis virus [J].