Francisella tularensis detection using magnetic labels and a magnetic biosensor based on frequency mixing

被引：38
作者：
Meyer, Martin H. F.
Krause, Hans-Joachim
Hartmann, Markus
Miethe, Peter
Oster, Juergen
Keusgen, Michael [1 ]
机构：
[1] Univ Marburg, Inst Pharmaceut Chem, D-35032 Marburg, Germany
[2] Res Ctr Juelich, Inst Bio & Nanosyst IBN2, Julich, Germany
[3] SENOVA GmbH, Jena, Germany
[4] Chemagen GmbH, Baesweiler, Germany
来源：
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2007年 / 311卷 / 01期
关键词：
Francisella tularensis; tularemia; magnetic bead; biosensor; magnetic label;
D O I：
10.1016/j.jmmm.2006.10.1175
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A biosensor that uses resonant coils with a special frequency-mixing technique and magnetic beads as detectable labels has been established for the detection of Francisella tularensis, the causative agent for tularemia. The detection principle is based on a sandwich immunoassay using an anti-Ft antibody for immunofiltration immobilized to ABICAP'(R) polyethylene filters, and biotinylated with streptavidin-coated magnetic beads as labels. The linear detection range of this biosensor was found to be 10(4)-10(6) cfu F tularensis lipopolysaccharide (LPS) per ml. Tested sample matrices were physiological PBS buffer and rabbit serum. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：259 / 263
页数：5
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