Francisella tularensis detection using magnetic labels and a magnetic biosensor based on frequency mixing

被引：38

作者：

Meyer, Martin H. F.

Krause, Hans-Joachim

Hartmann, Markus

Miethe, Peter

Oster, Juergen

Keusgen, Michael ^{[1
]}

机构：

[1] Univ Marburg, Inst Pharmaceut Chem, D-35032 Marburg, Germany

[2] Res Ctr Juelich, Inst Bio & Nanosyst IBN2, Julich, Germany

[3] SENOVA GmbH, Jena, Germany

[4] Chemagen GmbH, Baesweiler, Germany

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2007年 / 311卷 / 01期

关键词：

Francisella tularensis; tularemia; magnetic bead; biosensor; magnetic label;

D O I：

10.1016/j.jmmm.2006.10.1175

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A biosensor that uses resonant coils with a special frequency-mixing technique and magnetic beads as detectable labels has been established for the detection of Francisella tularensis, the causative agent for tularemia. The detection principle is based on a sandwich immunoassay using an anti-Ft antibody for immunofiltration immobilized to ABICAP'(R) polyethylene filters, and biotinylated with streptavidin-coated magnetic beads as labels. The linear detection range of this biosensor was found to be 10(4)-10(6) cfu F tularensis lipopolysaccharide (LPS) per ml. Tested sample matrices were physiological PBS buffer and rabbit serum. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：259 / 263

页数：5

共 23 条

[1]

[Anonymous], 2000, RU Patent, Patent No. [2166751, EP 1262766]

[2] Detection and manipulation of biomolecules by magnetic carriers [J].

Brzeska, M ;

Panhorst, M ;

Kamp, PB ;

Schotter, J ;

Reiss, G ;

Pühler, A ;

Becker, A ;

Brückl, H .

JOURNAL OF BIOTECHNOLOGY, 2004, 112 (1-2) :25-33

[3] Detection of biological threat agents by real-time PCR: Comparison of assay performance on the RAPID, the LightCycler, and the smart cycler platforms [J].

Christensen, DR ;

Hartman, LJ ;

Loveless, BM ;

Frye, MS ;

Shipley, MA ;

Bridge, DL ;

Richards, MJ ;

Kaplan, RS ;

Garrison, J ;

Baldwin, CD ;

Kulesh, DA ;

Norwood, DA .

CLINICAL CHEMISTRY, 2006, 52 (01) :141-145

[4] Tularemia as a biological weapon - Medical and public health management [J].

Dennis, DT ;

Inglesby, TV ;

Henderson, DA ;

Bartlett, JG ;

Ascher, MS ;

Eitzen, E ;

Fine, AD ;

Friedlander, AM ;

Hauer, J ;

Layton, M ;

Lillibridge, SR ;

McDade, JE ;

Osterholm, MT ;

O'Toole, T ;

Parker, G ;

Perl, TM ;

Russell, PK ;

Tonat, K .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2001, 285 (21) :2763-2773

[5] Planar Hall effect sensor for magnetic micro- and nanobead detection [J].

Ejsing, L ;

Hansen, MF ;

Menon, AK ;

Ferreira, HA ;

Graham, DL ;

Freitas, PP .

APPLIED PHYSICS LETTERS, 2004, 84 (23) :4729-4731

[6] Biodetection using magnetically labeled biomolecules and arrays of spin valve sensors (invited) [J].

Ferreira, HA ;

Graham, DL ;

Freitas, PP ;

Cabral, JMS .

JOURNAL OF APPLIED PHYSICS, 2003, 93 (10) :7281-7286

[7] Magnetic force-based multiplexed immunoassay using superparamagnetic nanoparticles in microfluidic channel [J].

Kim, KS ;

Park, JK .

LAB ON A CHIP, 2005, 5 (06) :657-664

[8]

KRAUSE HJ, 2004, Patent No. 2004077044

[9]

KRAUSE HJ, 2006, IN PRESS J MAGN MAGN

[10]

KRAUSE HJ, 2003, Patent No. 103091327

← 1 2 3 →