A disposable biosensor for the determination of alpha-amylase in human saliva

被引：34
作者：
Mahosenaho, Mika [2 ]
Caprio, Felice [1 ]
Micheli, Laura [1 ]
Sesay, Adama M. [2 ]
Palleschi, Giuseppe [1 ]
Virtanen, Vesa [2 ]
机构：
[1] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy
[2] Oulu Univ, Kajaani Univ Consortium, Biotechnol Lab, Sotkamo 88600, Finland
来源：
MICROCHIMICA ACTA | 2010年 / 170卷 / 3-4期
关键词：
Biosensor; Alpha amylase; Biomarker; Saliva; Stress; SYMPATHETIC ACTIVITY; ASCORBIC-ACID; STRESS; SYSTEM; CORTISOL; SENSOR; SERUM;
D O I：
10.1007/s00604-010-0360-y
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A disposable tri-enzymatic biosensor is presented for the determination of alpha-amylase in human saliva. It is based on the quantity of maltose generated by hydrolysis of maltopentose in the presence of salivary alpha-amylase. The biosensor is fabricated by co-immobilization of the enzymes alpha-glucosidase, glucose oxidase, and mutarotase on screen-printed electrodes modified with Prussian Blue. The assay can be performed with a "drop" of sample, this allowing for ease and simplicity. A linear relationship is found for the range from 5 to 250 units per mL, with an LOD of 5 units per mL. The biosensor is stable for at least one month and over this time retains 80% of its original activity. The system was then evaluated for matrix effects of human saliva and compared to a spectrometric method using a commercially available kit.
引用
收藏
页码：243 / 249
页数：7
相关论文
共 26 条
  • [1] Ascorbic acid-based inhibitors of α-amylases
    Abell, AD
    Ratcliffe, MJ
    Gerrard, J
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1998, 8 (13) : 1703 - 1706
  • [2] Stress as a determinant of saliva-mediated adherence and coadherence of oral and nonoral microorganisms
    Bosch, JA
    Turkenburg, M
    Nazmi, K
    Veerman, ECI
    de Geus, EJC
    Amerongen, AVN
    [J]. PSYCHOSOMATIC MEDICINE, 2003, 65 (04): : 604 - 612
  • [3] Salivary alpha-amylase as a measure of endogenous adrenergic activity
    Chatterton, RT
    Vogelsong, KM
    Lu, YC
    Ellman, AB
    Hudgens, GA
    [J]. CLINICAL PHYSIOLOGY, 1996, 16 (04): : 433 - 448
  • [4] DETERMINATION OF MALTASE AND ISOMALTASE ACTIVITIES WITH A GLUCOSE-OXIDASE REAGENT
    DAHLQVIST, A
    [J]. BIOCHEMICAL JOURNAL, 1961, 80 (03) : 547 - &
  • [5] EVIDENCE FOR ABSENCE OF ASCORBIC-ACID IN HUMAN SALIVA
    FELLER, RP
    BLACK, HS
    SHANNON, IL
    [J]. ARCHIVES OF ORAL BIOLOGY, 1975, 20 (09) : 563 - 566
  • [6] Salivary α-amylase in biobehavioral research -: Recent developments and applications
    Granger, Douglas A.
    Kivlighan, Katie T.
    el-Sheikh, Mona
    Gordis, Elana B.
    Stroud, Laura R.
    [J]. ORAL-BASED DIAGNOSTICS, 2007, 1098 : 122 - 144
  • [7] Salivary Glucose Concentration and Excretion in Normal and Diabetic Subjects
    Jurysta, Cedric
    Bulur, Nurdan
    Oguzhan, Berrin
    Satman, Ilhan
    Yilmaz, Temel M.
    Malaisse, Willy J.
    Sener, Abdullah
    [J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2009,
  • [8] Salivary alpha-amylase as a non-invasive biomarker for the sympathetic nervous system: Current state of research
    Nater, U. M.
    Rohleder, N.
    [J]. PSYCHONEUROENDOCRINOLOGY, 2009, 34 (04) : 486 - 496
  • [9] Human salivary alpha-amylase reactivity in a psychosocial stress paradigm
    Nater, UM
    Rohleder, N
    Gaab, J
    Berger, S
    Jud, A
    Kirschbaum, C
    Ehlert, U
    [J]. INTERNATIONAL JOURNAL OF PSYCHOPHYSIOLOGY, 2005, 55 (03) : 333 - 342
  • [10] Saliva collection method affects predictability of serum cortisol
    Poll, Eva-Maria
    Kreitschmann-Andermahr, Ilonka
    Langejuergen, Yvonne
    Stanzel, Sven
    Gilsbach, Joachim Michael
    Gressner, Axel
    Yagmur, Eray
    [J]. CLINICA CHIMICA ACTA, 2007, 382 (1-2) : 15 - 19
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