A disposable biosensor for the determination of alpha-amylase in human saliva

被引：34
作者：
Mahosenaho, Mika [2 ]
Caprio, Felice [1 ]
Micheli, Laura [1 ]
Sesay, Adama M. [2 ]
Palleschi, Giuseppe [1 ]
Virtanen, Vesa [2 ]
机构：
[1] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy
[2] Oulu Univ, Kajaani Univ Consortium, Biotechnol Lab, Sotkamo 88600, Finland
来源：
MICROCHIMICA ACTA | 2010年 / 170卷 / 3-4期
关键词：
Biosensor; Alpha amylase; Biomarker; Saliva; Stress; SYMPATHETIC ACTIVITY; ASCORBIC-ACID; STRESS; SYSTEM; CORTISOL; SENSOR; SERUM;
D O I：
10.1007/s00604-010-0360-y
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A disposable tri-enzymatic biosensor is presented for the determination of alpha-amylase in human saliva. It is based on the quantity of maltose generated by hydrolysis of maltopentose in the presence of salivary alpha-amylase. The biosensor is fabricated by co-immobilization of the enzymes alpha-glucosidase, glucose oxidase, and mutarotase on screen-printed electrodes modified with Prussian Blue. The assay can be performed with a "drop" of sample, this allowing for ease and simplicity. A linear relationship is found for the range from 5 to 250 units per mL, with an LOD of 5 units per mL. The biosensor is stable for at least one month and over this time retains 80% of its original activity. The system was then evaluated for matrix effects of human saliva and compared to a spectrometric method using a commercially available kit.
引用
收藏
页码：243 / 249
页数：7
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