Anti-HBV activity of the different extracts from Phyllanthus rheedei Wight in cell culture based assay systems

被引：5

作者：

Suresh, V ^{[1
]}

Jose, Sojan ^{[2
]}

Krishna, Radhika N. ^{[3
]}

Asha, V. V. ^{[1
]}

机构：

[1] Rajiv Gandhi Ctr Biotechnol, Trivandrum 695014, Kerala, India

[2] Govt Victoria Coll, Dept Bot, Palakkad 678001, Kerala, India

[3] Cent Tuber Crops Res Inst ICAR, Div Crop Improvement, Trivandrum 695017, Kerala, India

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2014年 / 156卷

关键词：

Phyllanthus rheedei Wight; Anti-HBV; Hepatitis; ELISA; SQRT-PCR; Immuno blotting; HEPATITIS-B-VIRUS; HUMAN HEPATOMA; INFECTION; HEPATOCYTES; PROTEINS; PLANTS;

D O I：

10.1016/j.jep.2014.08.028

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Phyllanthus rheedei Wight is a plant used by Muthuvan tribes of Kerala for treating liver related diseases. Materials and methods: The different extracts of Phyllanthus rheedei were analysed on cell lines were viz, PLC/PRF, Hep3B, FLCII10 and HepG2215 for its anti-HBV property. The analysis was done through ELISA, SQRT-PCR and immuno blotting. The most active extract was then divided in to fractions using HPTLC and the most active fraction was further identified. Results: From the screening experiments it was shown that the ethanol extract of this plant has the maximum activity in lowering the viral markers like HBsAg, HBV Core and HBV X protein and whole virions with comparatively lesser cytotoxicity. The dose responses of this particular extract were further established. Conclusions: This study concluded that the ethanol extract of Phyllanthus rheedei is very much effective in preventing the multiplication of HBV at the cellular level. This study scientifically validated the tribal claim of the use of this plant for severe liver disorders. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：309 / 315

页数：7

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