Direct detection of Borrelia burgdorferi in Ixodes scapularis (Acari: Ixodidae) nymphs by hybridization to ribosomal RNA

被引：0

作者：

Danishefsky, K ^{[1
]}

Ramachandra, N

Coleman, J

Schwartz, I

机构：

[1] Yeshiva Univ, Dept Biol, New York, NY 10033 USA

[2] New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 2000年 / 37卷 / 01期

关键词：

Borrelia burgdorferi; Ixodes scapularis; ribosomal RNA; Lyme disease; detection;

D O I：

10.1603/0022-2585-37.1.102

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A method for direct detection of Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner has been developed. Cells are lysed to facilitate release of ribosomal RNA. Lysates are filtered onto nylon membranes that are hybridized with probes specific for sequences in B. burgdorferi 23S rRNA. The technique is rapid and does not require any enzymatic amplification steps. With the use of a cocktail containing five different proles, approximate to 1,000 organisms could be detected. The assay was successfully applied to direct detection of B. burgdorferi in Ixodes scapularis Say nymphs.

引用

页码：102 / 107

页数：6

共 15 条

[1] THE BIOLOGICAL AND SOCIAL PHENOMENON OF LYME-DISEASE [J].