Recombinant protein expression in Pichia pastoris

被引：931
|
作者：
Cregg, JM
Cereghino, JL
Shi, JY
Higgins, DR
机构：
[1] Keck Grad Inst Appl Life Sci, Claremont, CA 91711 USA
[2] Oregon Grad Inst Sci & Technol, Beaverton, OR 97006 USA
[3] Idun Pharmaceut, La Jolla, CA 92037 USA
来源：
MOLECULAR BIOTECHNOLOGY | 2000年 / 16卷 / 01期
关键词：
Pichia pastoris; Pichia methanolica; methylotrophic yeast; heterologous protein production; foreign gene expression; yeast protein expression; fermentation;
D O I：
10.1385/MB:16:1:23
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool. What began more than 20 years ago as a program to convert abundant methanol to a protein source for animal feed has been developed into what is today two production system. To date well over 200 heterologous proteins have been expressed in P. pastoris. Significant advances in the development of new strains and vectors, improved techniques, and the commercial availability of these tools coupled with a better understanding of the biology of Pichia species have led to this microbe's value and power in commercial and research labs alike.
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收藏
页码：23 / 52
页数：30
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