Purification and characterization of a lectin from Annona coriacea seeds

被引：16
作者：
Coelho, MB
Freire, MD
Toyama, MH
Marangoni, S
Novello, JC
Macedo, MLR
机构：
[1] Univ Estadual Campinas, Inst Biol, Dept Bioquim, UNICAMP, Campinas, SP, Brazil
[2] Univ Fed Mato Grosso Sul, CEUL, Dept Ciencias Nat, Lab Purificacao Prot & Funcoes Biol, Tres Lagoas, MS USA
来源：
PROTEIN AND PEPTIDE LETTERS | 2003年 / 10卷 / 02期
关键词：
Annona coriacea; Annonaceae; amino acid composition; lectin; N-terminal sequence; seed;
D O I：
10.2174/0929866033479103
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel lectin, denominated ACLEC, was isolated from Annona coriacea seeds, belonging to the Annonaceae family. The lectin presented one protein band in SDS-PAGE of 14 kDa. Of the sugars tested, D-glucose and D-mannose were the best inhibitors. A search sequence database showed that ACLEC had homology with other plant lectins, belonging to leguminous lectin family.
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收藏
页码：165 / 173
页数：9
相关论文
共 29 条
[1]   Physicochemical characterization of Cajanus cajan lectin:: effect of pH and metal ions on lectin carbohydrate interaction [J].
Ahmad, S ;
Khan, RH ;
Ahmad, A .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1999, 1427 (03) :378-384
[2]   Purification and characterization of a lectin from seeds of Vatairea macrocarpa Duke [J].
Cavada, BS ;
Santos, CF ;
Grangeiro, TB ;
Nunes, EP ;
Sales, PVP ;
Ramos, RL ;
De Sousa, FAM ;
Crisostomo, CV ;
Calvete, JJ .
PHYTOCHEMISTRY, 1998, 49 (03) :675-680
[3]   LECTINS, LECTIN GENES, AND THEIR ROLE IN PLANT DEFENSE [J].
CHRISPEELS, MJ ;
RAIKHEL, NV .
PLANT CELL, 1991, 3 (01) :1-9
[4]   PURIFICATION AND CHARACTERIZATION OF 12 MAJOR LECTINS FROM ARAUCARIA-BRASILIENSIS SYN ARAUCARIA-ANGUSTIFOLIA SEEDS (PINHAO) [J].
DATTA, PK ;
FIGUEROA, MODCR ;
LAJOLO, FM .
PLANT PHYSIOLOGY, 1991, 97 (03) :856-862
[5]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[6]   Isolation and partial characterization of a novel lectin from Talisia esculenta seeds that interferes with fungal growth [J].
Freire, MDM ;
Gomes, VM ;
Corsini, RE ;
Machado, OLT ;
De Simone, SG ;
Novello, JC ;
Marangoni, S ;
Macedo, MLR .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2002, 40 (01) :61-68
[7]  
Gilg D, 1996, Pharm Acta Helv, V71, P383, DOI 10.1016/S0031-6865(96)00048-9
[8]  
HENRIKSON RL, 1984, ANAL BIOCHEM, V136, P65
[9]  
KEIICHI N, 1998, PHYTOCHEMISTRY, V49, P667
[10]   Mechanisms and assessment of lectin-mediated mitogenesis [J].
Kilpatrick, DC .
MOLECULAR BIOTECHNOLOGY, 1999, 11 (01) :55-65
123