High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

被引：18

作者：

Takemori, Nobuaki ^{[1
]}

Takemori, Ayako ^{[1
]}

Matsuoka, Kazuhiro ^{[1
,2
]}

Morishita, Ryo ^{[3
]}

Matsushita, Natsuki ^{[4
]}

Aoshima, Masato ^{[5
]}

Takeda, Hiroyuki ^{[1
,2
]}

Sawasaki, Tatsuya ^{[1
,2
]}

Endo, Yaeta ^{[2
]}

Higashiyama, Shigeki ^{[1
]}

机构：

[1] Ehime Univ, Proteosci Ctr, Matsuyama, Ehime 790, Japan

[2] Ehime Univ, Cell Free Sci & Technol Res Ctr, Matsuyama, Ehime, Japan

[3] CellFree Sci Co Ltd, Matsuyama, Ehime, Japan

[4] Ehime Univ Hosp, Translat Res Ctr, Matsuyama, Ehime, Japan

[5] KK AB SCIEX, Tokyo, Japan

来源：

MOLECULAR BIOSYSTEMS | 2015年 / 11卷 / 02期

关键词：

ABSOLUTE QUANTIFICATION; MEMBRANE-PROTEINS; FREE TRANSLATION; PURIFICATION; QUANTITATION; EXPRESSION; PEPTIDES; SUBUNITS;

D O I：

10.1039/c4mb00556b

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a wheat germcell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.

引用

页码：361 / 365

页数：5

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