LNA (Locked nucleic acid): High-affinity targeting of complementary RNA and DNA

被引：549

作者：

Vester, B ^{[1
]}

Wengel, J

机构：

[1] Univ So Denmark, Dept Biochem & Mol Biol, Nucle Acid Ctr, DK-5230 Odense M, Denmark

[2] Univ So Denmark, Dept Chem, DK-5230 Odense M, Denmark

来源：

BIOCHEMISTRY | 2004年 / 43卷 / 42期

关键词：

D O I：

10.1021/bi0485732

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double-stranded DNA. Structural studies have shown that LNA oligonucleotides induce A-type (RNA-like) duplex conformations. The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.

引用

页码：13233 / 13241

页数：9

共 60 条

[1] A structure-activity study of the inhibition of HIV-1 Tat-dependent trans-activation by mixmer 2′-O-methyl oligoribonucleotides containing locked nucleic acid (LNA), α-L-LNA, or 2′-thio-LNA residues [J].

Arzumanov, A ;

Stetsenko, DA ;

Malakhov, AD ;

Reichelt, S ;

Sorensen, MD ;

Babu, BR ;

Wengel, J ;

Gait, MJ .

OLIGONUCLEOTIDES, 2003, 13 (06) :435-453

[2] An autonomous molecular computer for logical control of gene expression [J].

Benenson, Y ;

Gil, B ;

Ben-Dor, U ;

Adar, R ;

Shapiro, E .

NATURE, 2004, 429 (6990) :423-429

[3] RNA interference in mammalian cells by chemically-modified RNA [J].

Braasch, DA ;

Jensen, S ;

Liu, YH ;

Kaur, K ;

Arar, K ;

White, MA ;

Corey, DR .

BIOCHEMISTRY, 2003, 42 (26) :7967-7975

[4] Recent improvements in antigene technology [J].

Buchini, S ;

Leumann, CJ .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (06) :717-726

[5] Oligonucleotide directed misfolding of RNA inhibits Candida albicans group I intron splicing [J].

Childs, JL ;

Disney, MD ;

Turner, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :11091-11096

[6] Design and characterization of decoy oligonucleotides containing locked nucleic acids [J].

Crinelli, R ;

Bianchi, M ;

Gentilini, L ;

Magnani, M .

NUCLEIC ACIDS RESEARCH, 2002, 30 (11) :2435-2443

[7] Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays [J].

Di Giusto, DA ;

King, GC .

NUCLEIC ACIDS RESEARCH, 2004, 32 (03) :e32

[8] A conformationally constrained nucleotide analogue controls the folding topology of a DNA G-quadruplex [J].

Dominick, PK ;

Jarstfer, MB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (16) :5050-5051

[9] Implications of high-affinity hybridization by locked nucleic acid oligomers for inhibition of human telomerase [J].

Elayadi, AN ;

Braasch, DA ;

Corey, DR .

BIOCHEMISTRY, 2002, 41 (31) :9973-9981

[10] Locked nucleic acid modified DNA enzymes targeting early growth response-1 inhibit human vascular smooth muscle cell growth [J].

Fahmy, RG ;

Khachigian, LM .

NUCLEIC ACIDS RESEARCH, 2004, 32 (07) :2281-2285

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