Imaging receptor microdomains on leukocyte subsets in live mice

被引：67

作者：

Chiang, Elaine Y.

Hidalgo, Andres

Chang, Jungshan

Frenette, Paul S.

机构：

[1] CUNY Mt Sinai Sch Med, Dept Med, New York, NY 10029 USA

[2] CUNY Mt Sinai Sch Med, Immunobiol Ctr, New York, NY 10029 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 03期

关键词：

D O I：

10.1038/nmeth1018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45(+) F4/80-Gr-1(+) neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45(+) B220(+) B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1(+) leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

引用

收藏

页码：219 / 222

页数：4

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