Oxysterols induce apoptosis and accumulation of cell cycle at G2/M phase in the human monocytic THP-1 cell line

被引：34
|
作者：
Lim, HK
Kang, HK
Yoo, ES
Kim, BJ
Kim, YW
Cho, M
Lee, JH
Lee, YS
Chung, MH
Hyun, JW
机构：
[1] Cheju Natl Univ, Dept Biochem, Coll Med, Jeju 690756, Jeju Do, South Korea
[2] Cheju Natl Univ, Dept Pharmacol, Coll Med, Jeju 690756, Jeju Do, South Korea
[3] Cheju Natl Univ, Dept Microbiol, Coll Med, Jeju 690756, Jeju Do, South Korea
[4] Cheju Natl Univ, Dept Pediat, Coll Med, Jeju 690756, Jeju Do, South Korea
[5] Silla Univ, Dept Biosci & Biotechnol, Pusan, South Korea
[6] Korea Canc Ctr Hosp, Lab Radiat Effect, Seoul, South Korea
[7] Seoul Natl Univ, Coll Med, Dept Pharmacol, Seoul, South Korea
来源：
LIFE SCIENCES | 2003年 / 72卷 / 12期
关键词：
oxysterol; apoptosis; cell cycle; cytotoxicity;
D O I：
10.1016/S0024-3205(02)02377-9
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The cytotoxic activity of oxysterols, 7beta-hydroxycholesterol (7beta-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols, showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G(2)/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7beta-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G(2)/M phase. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：1389 / 1399
页数：11
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