A simple method for obtaining a highly enantioselective enzymatic preparation from baker's yeast for the reduction of β-keto esters

被引：6
作者：
Menezes, RR [1 ]
Santos, AS [1 ]
Oestreicher, EG [1 ]
Pinto, GF [1 ]
机构：
[1] Fed Univ Rio De Janeiro, Inst Quim, Dept Bioquim, Ctr Tecnol, BR-21945900 Rio De Janeiro, Brazil
来源：
BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 01期
关键词：
D O I：
10.1023/A:1008899308479
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Baker's yeast beta-keto ester oxidoreductase was partially purified using anion exchange chromatography (DEAE-Sephacell) and was enantioselective for the production of ethyl (S)-(alpha)-beta-hydroxybutanoate and suitable for kinetic studies.
引用
收藏
页码：35 / 37
页数：3
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