Synthesis of Eco-Friendly Silver Nanoparticles Using Glycyrrhizin and Evaluation of Their Antibacterial Ability

被引:29
作者
Feng, Danni [1 ]
Zhang, Renyin [1 ]
Zhang, Mengting [1 ]
Fang, Ashe [1 ]
Shi, Feng [1 ]
机构
[1] Shihezi Univ, Coll Life Sci, Shihezi 832003, Peoples R China
基金
中国国家自然科学基金;
关键词
silver nanoparticles; glycyrrhizin; antibacterial; green synthesis; ESCHERICHIA-COLI; GREEN SYNTHESIS; EXTRACT; SHAPE;
D O I
10.3390/nano12152636
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In the present study, the biosynthesis of silver nanoparticles (AgNPs) and their antibacterial activity against gram-positive and gram-negative bacteria were investigated. Glycyrrhizin (GL) was used as a reducing agent and stabilizer to rapidly prepare the AgNPs. The distinctive absorption peak at 419 nm confirmed the formation of GL-reduced AgNPs. The TEM and particle size analysis shows that the prepared GL-reduced AgNPs were mostly circular with good dispersion and a relatively uniform particle size of 35 nm on average. Fourier transform infrared spectroscopy analysis was performed to identify the possible biomolecules in the capping and active stabilization of the GL-reduced AgNPs. The antibacterial activity of the GL-reduced AgNPs was analyzed with the Oxford cup diffusion method and filter paper diffusion method. The experimental results show that these properties endowed the GL-reduced AgNPs with high antibacterial activity against Escherichia coli and Staphylococcus aureus and lay a foundation for the use of colloidal silver in antibacterial applications. The GL-reduced AgNPs also had stronger antibacterial activity than sodium citrate-reduced AgNPs, which indicates the advantages of GL-reduced AgNPs compared with sodium citrate-reduced AgNPs in inducing bacteriostasis. The cytotoxicity of GL-reduced AgNPs on human kidney epithelial 293A (HEK293) cells was evaluated via the MTT assay. The results show that GL-reduced AgNPs had lower toxicity to HEK293 cells than sodium citrate-AgNPs, which indicates that the as-prepared GL-reduced AgNPs are environmentally friendly.
引用
收藏
页数:12
相关论文
共 30 条
[11]  
Gurunathan S, 2014, J GENET ENV RESOUR C, V9, P373
[12]   Green Synthesis of Silver Nanoparticles Using Pinus eldarica Bark Extract [J].
Iravani, Siavash ;
Zolfaghari, Behzad .
BIOMED RESEARCH INTERNATIONAL, 2013, 2013
[13]   Antimicrobial effects of silver nanoparticles [J].
Kim, Jun Sung ;
Kuk, Eunye ;
Yu, Kyeong Nam ;
Kim, Jong-Ho ;
Park, Sung Jin ;
Lee, Hu Jang ;
Kim, So Hyun ;
Park, Young Kyung ;
Park, Yong Ho ;
Hwang, Cheol-Yong ;
Kim, Yong-Kwon ;
Lee, Yoon-Sik ;
Jeong, Dae Hong ;
Cho, Myung-Haing .
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2007, 3 (01) :95-101
[14]   Nasal carriage of Staphylococcus aureus: Epidemiology, underlying mechanisms, and associated risks [J].
Kluytmans, J ;
vanBelkum, A ;
Verbrugh, H .
CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (03) :505-+
[15]   ADSORPTION AND SURFACE-ENHANCED RAMAN OF DYES ON SILVER AND GOLD SOLS [J].
LEE, PC ;
MEISEL, D .
JOURNAL OF PHYSICAL CHEMISTRY, 1982, 86 (17) :3391-3395
[16]   Shape and aggregation control of nanoparticles: Not shaken, not stirred [J].
Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095-1569, United States .
J. Am. Chem. Soc., 2006, 3 (968-975)
[17]   Bio-fabricated silver nanoparticles preferentially targets Gram positive depending on cell surface charge [J].
Mandal, Debasis ;
Dash, Sandeep Kumar ;
Das, Balaram ;
Chattopadhyay, Sourav ;
Ghosh, Totan ;
Das, Debasis ;
Roy, Somenath .
BIOMEDICINE & PHARMACOTHERAPY, 2016, 83 :548-558
[18]  
Miri ST, 2017, IRAN J MICROBIOL, V9, P33
[20]   Comparative synthesis and antimicrobial action of silver nanoparticles and silver nitrate [J].
Mosselhy, Dina A. ;
El-Aziz, Mohamed Abd ;
Hanna, Magdy ;
Ahmed, Mohamed A. ;
Husien, Mona M. ;
Feng, Qingling .
JOURNAL OF NANOPARTICLE RESEARCH, 2015, 17 (12) :1-10