Synthesis of Eco-Friendly Silver Nanoparticles Using Glycyrrhizin and Evaluation of Their Antibacterial Ability

被引:29
作者
Feng, Danni [1 ]
Zhang, Renyin [1 ]
Zhang, Mengting [1 ]
Fang, Ashe [1 ]
Shi, Feng [1 ]
机构
[1] Shihezi Univ, Coll Life Sci, Shihezi 832003, Peoples R China
基金
中国国家自然科学基金;
关键词
silver nanoparticles; glycyrrhizin; antibacterial; green synthesis; ESCHERICHIA-COLI; GREEN SYNTHESIS; EXTRACT; SHAPE;
D O I
10.3390/nano12152636
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In the present study, the biosynthesis of silver nanoparticles (AgNPs) and their antibacterial activity against gram-positive and gram-negative bacteria were investigated. Glycyrrhizin (GL) was used as a reducing agent and stabilizer to rapidly prepare the AgNPs. The distinctive absorption peak at 419 nm confirmed the formation of GL-reduced AgNPs. The TEM and particle size analysis shows that the prepared GL-reduced AgNPs were mostly circular with good dispersion and a relatively uniform particle size of 35 nm on average. Fourier transform infrared spectroscopy analysis was performed to identify the possible biomolecules in the capping and active stabilization of the GL-reduced AgNPs. The antibacterial activity of the GL-reduced AgNPs was analyzed with the Oxford cup diffusion method and filter paper diffusion method. The experimental results show that these properties endowed the GL-reduced AgNPs with high antibacterial activity against Escherichia coli and Staphylococcus aureus and lay a foundation for the use of colloidal silver in antibacterial applications. The GL-reduced AgNPs also had stronger antibacterial activity than sodium citrate-reduced AgNPs, which indicates the advantages of GL-reduced AgNPs compared with sodium citrate-reduced AgNPs in inducing bacteriostasis. The cytotoxicity of GL-reduced AgNPs on human kidney epithelial 293A (HEK293) cells was evaluated via the MTT assay. The results show that GL-reduced AgNPs had lower toxicity to HEK293 cells than sodium citrate-AgNPs, which indicates that the as-prepared GL-reduced AgNPs are environmentally friendly.
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页数:12
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