Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis

被引：9

作者：

Chen, Dongsheng ^{[1
,2
]}

Zhu, Xiangxiang ^{[1
]}

Zhou, Lijuan ^{[1
]}

Wang, Jian ^{[1
]}

Tao, Xiaoqian ^{[1
]}

Wang, Shuang ^{[1
]}

Sun, Fuling ^{[1
]}

Kan, Xianzhao ^{[1
,2
]}

Han, Zhengqi ^{[1
]}

Gu, Yuelin ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Life Sci, Prov Key Lab Conservat & Exploitat Res Biol Resou, Wuhu 241000, Peoples R China

[2] Anhui Normal Univ, Inst Bioinformat, Coll Life Sci, Wuhu 241000, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

美国国家科学基金会;

关键词：

BAM TRANSCRIPTION; RT-PCR; PROTEIN; DPP; MELANOGASTER; DETERMINES; ACTIVATION; DAUGHTERS; DIVISION; REVEALS;

D O I：

10.1038/s41598-017-05975-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Emerging evidence supports that stem cells are regulated by both intrinsic and extrinsic mechanisms. However, factors that determine the fate of stem cells remain incompletely understood. The Drosophila testis provides an exclusive powerful model in searching for potential important regulatory factors and their underlying mechanisms for controlling the fate of germline stem cells (GSCs). In this study, we have found that Drosophila gilgamesh (gish), which encodes a homologue of human CK1-gamma (casein kinase 1-gamma), is required intrinsically for GSC maintenance. Our genetic analyses indicate gish is not required for Dpp/Gbb signaling silencing of bam and is dispensable for Dpp/Gbb signaling-dependent Dad expression. Finally, we show that overexpression of gish fail to dramatically increase the number of GSCs. These findings demonstrate that gish controls the fate of GSCs in Drosophila testis by a novel Dpp/Gbb signaling-independent pathway.

引用

页数：9

共 39 条

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