Separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography

被引：3
|
作者：
Leytem, AB [1 ]
Mikkelsen, RL
机构：
[1] USDA ARS, NWISRL, Kimberly, ID 83341 USA
[2] Potash & Phosphate Inst, Davis, CA USA
来源：
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS | 2003年 / 34卷 / 9-10期
关键词：
D O I：
10.1081/CSS-120020452
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Methods were developed for the extraction and separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography (RP-ICP). Nucleotides (ATP, ADP, and AMP) were separated using 4 mobile phase of 15 mM TBAHS, 15 mM KH2PO4, and 7% acetonitrile. Inositol hexakisphosphate was separated using a mobile phase composition of 0.05 M formic acid:methanol (49:51 v/v) and 1.5 mL/100 mL of TBAOH. Extraction procedures were developed for the nucleotides which would be compatible with the RP-ICP system developed for their separation.
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页码：1393 / 1406
页数：14
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