Separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography

被引：3
|
作者：
Leytem, AB [1 ]
Mikkelsen, RL
机构：
[1] USDA ARS, NWISRL, Kimberly, ID 83341 USA
[2] Potash & Phosphate Inst, Davis, CA USA
来源：
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS | 2003年 / 34卷 / 9-10期
关键词：
D O I：
10.1081/CSS-120020452
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Methods were developed for the extraction and separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography (RP-ICP). Nucleotides (ATP, ADP, and AMP) were separated using 4 mobile phase of 15 mM TBAHS, 15 mM KH2PO4, and 7% acetonitrile. Inositol hexakisphosphate was separated using a mobile phase composition of 0.05 M formic acid:methanol (49:51 v/v) and 1.5 mL/100 mL of TBAOH. Extraction procedures were developed for the nucleotides which would be compatible with the RP-ICP system developed for their separation.
引用
收藏
页码：1393 / 1406
页数：14
相关论文
共 50 条
  • [31] REVERSED-PHASE ION-PAIR CHROMATOGRAPHY OF DRUGS AND RELATED ORGANIC-COMPOUNDS
    JOHANSSON, IM
    WAHLUND, KG
    SCHILL, G
    JOURNAL OF CHROMATOGRAPHY, 1978, 149 (FEB): : 281 - 296
  • [32] Simultaneous determination of silicon and phosphorus in soil and plants by reversed-phase ion-pair chromatography
    Hou, Shengjie
    Ding, Mingyu
    Zhu, Jiang
    TALANTA, 2008, 75 (01) : 178 - 182
  • [33] ANYL 129-Novel mobile phase additives for the separation of both inorganic and organic compounds by ion-pair chromatography
    Zhong, Yingying
    Zhu, Yan
    Chen, Mei-Lan
    Fan, Yunchang, Sr.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2008, 236
  • [34] DETERMINATION OF CEFMENOXIME IN HUMAN-SERUM BY ION-PAIR REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    SMITH, IL
    SWANSON, DJ
    WELAGE, LS
    DEANGELIS, C
    BOUDINOT, SA
    SCHENTAG, JJ
    ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1985, 18 (09): : 1077 - 1085
  • [35] Ion-pair reverse-phase high performance liquid chromatography method for determination of Huperzine-A in beagle dog serum
    Ye, JC
    Zeng, S
    Zhang, WG
    Chen, GS
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 817 (02): : 187 - 191
  • [36] Quantification of ssDNA Scaffold Production by Ion-Pair Reverse Phase Chromatography
    Silva-Santos, A. Rita
    Rosa, Sara Sousa
    Marques, Marco P. C.
    Azevedo, Ana M.
    Prazeres, Duarte Miguel F.
    ACS OMEGA, 2024, 9 (21): : 22619 - 22624
  • [37] SEPARATION OF INORGANIC ANIONS BY ION-PAIR, REVERSE PHASE LIQUID-CHROMATOGRAPHY MONITORED BY INDIRECT PHOTOMETRY
    DREUX, M
    LAFOSSE, M
    PEQUIGNOT, M
    CHROMATOGRAPHIA, 1982, 15 (10) : 653 - 656
  • [38] DIRECT SEPARATION OF ENANTIOMERS USING CHIRAL ION-PAIR CHROMATOGRAPHY
    KARLSSON, A
    ACTA PHARMACEUTICA NORDICA, 1992, 4 (01): : 63 - 64
  • [39] Determination of iodine in organic compounds by ion-pair chromatography with C30 as a stationary phase
    Hiraki, N
    Isozaki, A
    Nagashima, H
    BUNSEKI KAGAKU, 2005, 54 (02) : 143 - 147
  • [40] High-throughput analysis of nucleic acid modification reactions using ion-pair reverse-phase high-performance liquid chromatography
    Dickman, MJ
    Matin, MM
    Hornby, DP
    ANALYTICAL BIOCHEMISTRY, 2002, 301 (02) : 290 - 297
12345