Low and heterogeneous prevalence of glucose-6-phosphate dehydrogenase deficiency in different settings in Ethiopia using phenotyping and genotyping approaches

被引:8
作者
Shitaye, Getasew [1 ]
Gadisa, Endalamaw [2 ]
Grignard, Lynn [3 ]
Shumie, Girma [2 ]
Chali, Wakweya [2 ]
Menberu, Temesgen [2 ]
Belachew, Mulualem [4 ]
Tegegn, Getaneh [5 ]
Challi, Sagni [2 ]
Curry, Jonathan [6 ]
Mahey, Laleta [6 ]
Hailu, Tsegaye [2 ]
Mamo, Hassen [7 ]
Menon, Menakath [8 ]
Balcha, Taye [2 ]
Aseffa, Abraham [2 ]
Drakeley, Chris [3 ]
Bousema, Teun [3 ,9 ]
Tadesse, Fitsum G. [2 ,9 ,10 ]
机构
[1] Bahir Dar Univ, Sch Med Sci, Dept Biomed Sci, Bahir Dar, Ethiopia
[2] Armauer Hansen Res Inst, Addis Ababa, Ethiopia
[3] London Sch Hyg & Trop Med, Dept Immunol & Infect, London, England
[4] Wolkite Univ, Sch Med Sci, Dept Biomed Sci, Wolkite, Ethiopia
[5] Semera Univ, Dept Biomed Sci, Semera, Ethiopia
[6] LGC Grp Ltd, Genom Div, Hoddesdon, Herts, England
[7] Addis Ababa Univ, Coll Nat Sci, Dept Microbial Cellular & Mol Biol, Addis Ababa, Ethiopia
[8] Addis Ababa Univ, Sch Med Sci, Dept Biochem, Addis Ababa, Ethiopia
[9] Radboud Univ Nijmegen, Med Ctr, Radboud Inst Hlth Sci, Nijmegen, Netherlands
[10] Addis Ababa Univ, Inst Biotechnol, POB 109, Addis Ababa, Ethiopia
基金
欧洲研究理事会; 比尔及梅琳达.盖茨基金会;
关键词
Radical cure; P; vivax; G6PD; 8-Aminoquinoline; Haemolysis; PLASMODIUM-VIVAX; MALARIA; FALCIPARUM; PRIMAQUINE; VARIANTS; CARRIAGE; GENE;
D O I
10.1186/s12936-018-2437-8
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: 8-Aminoquinolines such as primaquine clear mature Plasmodium falciparum gametocytes that are responsible for transmission from human to mosquitoes and bring radical cure in Plasmodium vivax by clearing dormant liver stages. Deployment of primaquine is thus of relevance for malaria elimination efforts but challenged by the widespread prevalence of glucose-6-phosphate dehydrogenase deficiency (G6PDd) in endemic countries since primaquine in G6PDd individuals may lead to acute haemolysis. In this study, the prevalence of G6PDd was investigated in different settings in Ethiopia using phenotyping and genotyping approaches. Methods: Community and school based cross-sectional surveys were conducted from October to December 2016 in four administrative regions (Gambela, Benishangul Gumuz, Oromia, and Amhara) in Ethiopia. Finger prick blood samples were collected for G6PD enzyme activity using the CareStart (TM) G6PD screening test and genotyping of 36 selected single nucleotide polymorphisms (SNPs) located in the G6PD gene and its flanking regions. Results: Overall, the prevalence of phenotypic G6PDd was 1.4% (22/1609). For the first time in the Ethiopian population, the African variant (A-) was detected in 3.5% (7/199) of the limited set of genotyped samples, which were all phenotypically normal. Interestingly, all of these individuals had a variation at the rs2515904 locus. Strong geographical variation was observed for both phenotypic and genotypic G6PDd; three-quarters of the phenotypically G6PDd individuals were detected in Gambela. Conclusion: A very low prevalence of G6PDd was detected in the present study populations. The presence of the A-variant alongside other G6PD mutants and the patchy distribution of G6PDd indicate that larger studies specifically designed to unravel the distribution of G6PDd at small geographical scale may be needed to tailor malaria elimination efforts in Ethiopia to the local context.
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页数:11
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