Statherin-derived peptides as antifungal strategy against Candida albicans

被引：7

作者：

Guimaraes Pellissari, Claudia Viviane ^{[1
,2
]}

Jorge, Janaina Habib ^{[1
]}

Marin, Lina Maria ^{[2
]}

Sabino-Silva, Robinson ^{[3
]}

Siqueira, Walter Luiz ^{[2
]}

机构：

[1] Sao Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, UNESP, 1680 Rua Humaita, BR-14801385 Araraquara, SP, Brazil

[2] Univ Saskatchewan, Coll Dent, 105 Wiggins Rd, Saskatoon, SK S7N 5E4, Canada

[3] Univ Fed Uberlandia, Inst Biomed Sci, Dept Physiol, 3Q-2121 Av Joao Naves de Avila, BR-38400902 Uberlandia, MG, Brazil

来源：

ARCHIVES OF ORAL BIOLOGY | 2021年 / 125卷

基金：

巴西圣保罗研究基金会; 加拿大健康研究院;

关键词：

Antifungal activity; Candida albicans; Stomatitis; WHOLE SALIVA; HYDROXYAPATITE; COLONIZATION; PROTEOLYSIS; INVASION; SYSTEMS;

D O I：

10.1016/j.archoralbio.2021.105106

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: The aim of this in vitro study was to evaluate the effect of statherin and its naturally occurring peptides (DR9-2, DR9, GE-12, IT-32, GQ-19, IP-18) on Candida albicans metabolism and biofilm development. Design: After the killing assay, a peptide pellicle was formed on the bottom of a polystyrene plate at the IC50 of each peptide. Over the peptide pellicle, Candida albicans biofilm (48 h) was grown. The peptides antimicrobial activity after the peptides treatment was evaluated by alamarBlue, total biofilm biomass and colony forming units (CFU) counting. Results: The pellicle with statherin and the peptides (DR9-2, DR9, GE-12, IP-18, GQ-19) was able to reduce he viability of Candida albicans compared to the negative control. They also decreased cell proliferation by 20 % and total biomass. IT-32 showed the highest reduction in cell proliferation and biomass, which was similar to the positive control, histatin 5. Conclusions: These results suggest that the naturally occuring peptides from statherin are able to decrease Candida albicans colonization and biofilm proliferation.

引用

页数：5

共 30 条

[1] Candida albicans promotes invasion and colonisation of Candida glabrata in a reconstituted human vaginal epithelium [J].

Alves, Carlos Tiago ;

Wei, Xiao-Qing ;

Silva, Sonia ;

Azeredo, Joana ;

Henriques, Mariana ;

Williams, David W. .

JOURNAL OF INFECTION, 2014, 69 (04) :396-407

[2] Elucidating role of salivary proteins in denture stomatitis using a proteomic approach [J].

Bencharit, Sompop ;

Altarawneh, Sandra K. ;

Baxter, Sarah Schwartz ;

Carlson, Jim ;

Ross, Gary F. ;

Border, Michael B. ;

Mack, C. Russell ;

Byrd, Warren C. ;

Dibble, Christopher F. ;

Barros, Silvana ;

Loewy, Zvi ;

Offenbacher, Steven .

MOLECULAR BIOSYSTEMS, 2012, 8 (12) :3216-3223

[3] Antifungal resistance of candidal biofilms formed on denture acrylic in vitro [J].

Chandra, J ;

Mukherjee, PK ;

Leidich, SD ;

Faddoul, FF ;

Hoyer, LL ;

Douglas, LJ ;

Ghannoum, MA .

JOURNAL OF DENTAL RESEARCH, 2001, 80 (03) :903-908

[4] D-Cateslytin: a new antifungal agent for the treatment of oral Candida albicans associated infections [J].

Dartevelle, Pauline ;

Ehlinger, Claire ;

Zaet, Abdurraouf ;

Boehler, Christian ;

Rabineau, Morgane ;

Westermann, Benoit ;

Strub, Jean-Marc ;

Cianferani, Sarah ;

Haikel, Youssef ;

Metz-Boutigue, Marie-Helene ;

Marban, Celine .

SCIENTIFIC REPORTS, 2018, 8

[5] Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology [J].

Dias Panariello, Beatriz Helena ;

Klein, Marlise I. ;

Pavarina, Ana Claudia ;

Duarte, Simone .

JOURNAL OF ORAL MICROBIOLOGY, 2017, 9

[6] Characterization of Mucosal Candida albicans Biofilms [J].

Dongari-Bagtzoglou, Anna ;

Kashleva, Helena ;

Dwivedi, Prabhat ;

Diaz, Patricia ;

Vasilakos, John .

PLOS ONE, 2009, 4 (11)

[7] G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences [J].

Faul, Franz ;

Erdfelder, Edgar ;

Lang, Albert-Georg ;

Buchner, Axel .

BEHAVIOR RESEARCH METHODS, 2007, 39 (02) :175-191

[8] Candida albicans morphogenesis and host defence: discriminating invasion from colonization [J].

Gow, Neil A. R. ;

van de Veerdonk, Frank L. ;

Brown, Alistair J. P. ;

Netea, Mihai G. .

NATURE REVIEWS MICROBIOLOGY, 2012, 10 (02) :112-122

[9] Oral fluid proteolytic effects on histatin 5 structure and function [J].

Helmerhorst, E. J. ;

Alagl, A. S. ;

Siqueira, W. L. ;

Oppenheim, F. G. .

ARCHIVES OF ORAL BIOLOGY, 2006, 51 (12) :1061-1070

[10] Whole saliva proteolysis - Wealth of information for diagnostic exploitation [J].

Helmerhorst, Eva J. .

ORAL-BASED DIAGNOSTICS, 2007, 1098 :454-460

← 1 2 3 →