Oxidation of 1-hydroxybenzotriazole by laccase and lignin peroxidase

被引：79
|
作者：
Ander, P [1 ]
Messner, K [1 ]
机构：
[1] Univ Technol Vienna, Dept Mycol, Inst Biochem Technol & Microbiol, A-1060 Vienna, Austria
来源：
BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 03期
关键词：
D O I：
10.1023/A:1008813206178
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method to measure laccase and lignin peroxidase (LiP) activity at 408 nm (402-410 nm) using 1-hydroxybenzotriazole (HBT) was developed. The assay can be performed either as a kinetic measurement or as a stopped reaction using 5 mM Na-azide which improves the spectrum. Only white-rot fungal laccases and LiP were found to oxidize HBT to give shoulders or peaks at 402-410 nm. Phanerochaete and Phlebia manganese peroxidases did not give absorbance increase at 402-410 nm.
引用
收藏
页码：191 / 195
页数：5
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